Impact of Caffeine and/or Estrogen Deficiency on Trabecular Bone Area and Healing: A Study in Rats

Autor(es)

Bastos, Marta Ferreira ^[1] ; Menezes, Diogo Jose Barreto ^[1] ; Bezerra, Joyce Pinho ^[1] ; Craneck Braz, Caetlin Kelmy ^[1] ; Silva Fonseca, Paula Fernanda ^[1] ; Arana-Chavez, Victor Elias ^[2] ; Azambuja, Nilton ^[3] ; Duarte, Poliana Mendes ^[1]

Afiliação do(s) autor(es)

^[1] Univ Guarulhos, Dent Res Div, Dept Periodont, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Dent, Dept Biomat & Oral Biol, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Dent, Dept Endodont, Basic Res Lab, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF ORAL & MAXILLOFACIAL IMPLANTS; v. 29, n. 1, p. 221-231, JAN-FEB 2014.
• Citações Web of Science: 1

Resumo

Purpose: To evaluate the effects of caffeine and/or estrogen deficiency on trabecular bone area (TBA) and bone healing in rats. Materials and Methods: Rats were divided into groups (n = 15/group) as follows: control, caffeine, ovariectomy (OVX), and caffeine/OVX. Critical-sized defects were created in the tibiae (57 days after beginning caffeine administration and 43 days after OVX). The intact femurs were evaluated for TBA and the number of positive cells for tartrate-resistant acid phosphatase (TRAP), receptor activator of nuclear factor-kappa B ligand (RANKL), and osteoprotegerin (OPG). In the defects, bone healing, the number of TRAP+ and RANKL/OPG+ cells, and gene expression of bone morphogenetic protein (BMP)-2, BMP-7, osteopontin, and CBP/p300-interacting-transactivator-with-ED-rich-tail-2 (CITED-2) were evaluated. Results: Bone healing was poorer in defects of the caffeine group than in those of the control group. The femurs of the OVX and OVX/caffeine groups presented lower TBAs and higher RANKL/OPG+ cell ratios. The number of TRAP+ cells was higher in femurs of the caffeine group and in defects of the OVX group. The caffeine/OVX group presented the highest RANKL/OPG+ cell ratio in femurs and defects. The OVX group presented the highest expression of BMP-2, BMP-7, and CITED-2. Conclusion: Caffeine affected bone healing, while estrogen deficiency mainly affected TBA, but no significant deleterious synergic effects of both conditions were observed. (AU)

• Processo FAPESP: 09/09446-6 - Impacto da ingestão crônica de cafeína associada ou não a osteoporose na perda óssea alveolar induzida por ligadura, na densidade e reparo ósseo em ratas
• Beneficiário: Marta Ferreira Bastos
• Modalidade de apoio: Auxílio à Pesquisa - Regular