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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Structure and Mechanism of Dimer-Monomer Transition of a Plant Poly(A)-Binding Protein upon RNA Interaction: Insights into Its Poly(A) Tail Assembly

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Autor(es):
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Domingues, Mariane Noronha [1] ; Sforca, Mauricio Luis [1] ; Soprano, Adriana Santos [1] ; Lee, Jack [2] ; Campos Brasil de Souza, Tatiana de Arruda [3] ; Cassago, Alexandre [4] ; Portugal, Rodrigo Villares [4] ; de Mattos Zeri, Ana Carolina [1] ; Murakami, Mario Tyago [1] ; Sadanandom, Ari [2] ; Lopes de Oliveira, Paulo Sergio [1] ; Benedetti, Celso Eduardo [1]
Número total de Autores: 12
Afiliação do(s) autor(es):
[1] Ctr Nacl Pesquisa Energia & Mat, Lab Nacl Biociencias, Campinas, SP - Brazil
[2] Univ Durham, Sch Biol & Biomed Sci, Durham DH1, Durham - England
[3] Fundacao Oswaldo Cruz, Inst Carlos Chagas, BR-81350010 Curitiba, Parana - Brazil
[4] Ctr Nacl Pesquisa Energia & Mat, Lab Nacl Nanotecnol, Campinas, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Journal of Molecular Biology; v. 427, n. 15, p. 2491-2506, JUL 31 2015.
Citações Web of Science: 4
Resumo

Poly(A)-binding proteins (PABPs) play crucial roles in mRNA biogenesis, stability, transport and translational control in most eukaryotic cells. Although animal PABPs are well-studied proteins, the biological role, three-dimensional structure and RNA-binding mode of plant PABPs remain largely uncharacterized. Here, we report the structural features and RNA-binding mode of a Citrus sinensis PABP (CsPABPN1). CsPABPN1 has a domain architecture of nuclear PABPs (PABPNs) with a single RNA recognition motif (RRM) flanked by an acidic N-terminus and a GRPF-rich C-terminus. The RRM domain of CsPABPN1 displays virtually the same three-dimensional structure and poly(A)-binding mode of animal PABPNs. However, while the CsPABPN1 RRM domain specifically binds poly(A), the full-length protein also binds poly(U). CsPABPN1 localizes to the nucleus of plant cells and undergoes a dimer-monomer transition upon poly(A) interaction. We show that poly(A) binding by CsPABPN1 begins with the recognition of the RNA-binding sites RNP1 and RNP2, followed by interactions with residues of the 132 strands, which stabilize the dimer, thus leading to dimer dissociation. Like human PABPN1, CsPABPN1 also seems to form filaments in the presence of poly(A). Based on these data, we propose a structural model in which contiguous CsPABPN1 RRM monomers wrap around the RNA molecule creating a superhelical structure that could not only shield the poly(A) tail but also serve as a scaffold for the assembly of additional mRNA processing factors. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 11/20468-1 - Modelos biológicos de interação planta-patógeno para o entendimento de mecanismos de patogenicidade e adaptação de fitobactérias, respostas de defesa e desenvolvimento de doença em citros
Beneficiário:Celso Eduardo Benedetti
Linha de fomento: Auxílio à Pesquisa - Temático