Clinical characteristics of women diagnosed with carcinoma who tested positive for cervical and anal high-risk human papillomavirus DNA and E6 RNA

Autor(es):Mostrar menos -

Veo, Carlos A. R. ^[1] ; Saad, Sarhan S. ^[2] ; Fregnani, Jose Humberto T. G. ^{[1, 3]} ; Scapulatempo-Neto, Cristovam ^[1] ; Tsunoda, Audrey Tieko ^{[1, 3]} ; Possati Resende, Julio Cesar ^[1] ; Lorenzi, Adriana Tarla ; Mafra, Allini ^[1] ; Cinti, Claudia ^[4] ; Cotrim, Ismael Dale ^[4] ; Reis Rosa, Luciana Albina ^[5] ; de Oliveira, Cristina Mendes ^[5] ; Martins, Toni Ricardo ^[5] ; Centrone, Cristiane ^[5] ; Levi, Jose Eduardo ^[5] ; Longatto-Filho, Adhemar ^{[6, 7, 3, 8]}

Afiliação do(s) autor(es)

^[1] Pio XII Fdn, Barretos Canc Hosp, Barretos - Brazil

^[2] Fed Univ Sao Paulo UNIFESP, Sch Med, Dept Gastroenterol, Sao Paulo - Brazil

^[3] Pio XII Fdn, Barretos Canc Hosp, Mol Oncol Res Ctr, Barretos - Brazil

^[4] Univ Fed Sao Paulo, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Inst Trop Med, Virol Lab, Sao Paulo - Brazil

^[6] Univ Minho, Sch Hlth Sci, Life & Hlth Sci Res Inst ICVS, Braga - Portugal

^[7] ICVS 3Bs PT Govt Associate Lab, Braga - Portugal

^[8] Univ Sao Paulo, Fac Med, Lab Med Invest LIM 14, BR-1246903 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: TUMOR BIOLOGY; v. 36, n. 7, p. 5399-5405, JUL 2015.
• Citações Web of Science: 5

Resumo

High-risk human papillomavirus (hrHPV) is an essential cause of cervical carcinoma and is also strongly related to anal cancer development. The hrHPV E6 oncoprotein plays a major role in carcinogenesis. We aimed to evaluate the frequency of hrHPV DNA and E6 oncoprotein in the anuses of women with cervical carcinoma. We analyzed 117 women with cervical cancer and 103 controls for hrHPV and the E6 oncogene. Positive test results for a cervical carcinoma included 66.7 % with hrHPV-16 and 7.7 % with hrHPV-18. One case tested positive for both HPV variants (0.9 %). The samples from the anal canal were positive for HPV-16 in 59.8 % of the cases. Simultaneous presence of HPV in the cervix and anal canal was found in 53.8 % of the cases. Regarding expression of E6 RNA, positivity for HPV-16 in the anal canal was found in 21.2 % of the cases, positivity for HPV-16 in the cervix was found in 75.0 %, and positivity for HPV-18 in the cervix was found in 1.9 %. E6 expression in both the cervix and anal canal was found in 19.2 % of the cases. In the controls, 1 % tested positive for HPV-16 and 0 % for HPV-18. Anal samples from the controls showed a hrHPV frequency of 4.9 % (only HPV16). The presence of hrHPV in the anal canal of women with cervical cancer was detected at a high frequency. We also detected E6 RNA expression in the anal canal of women with cervical cancer, suggesting that these women are at risk for anal hrHPV infection. (AU)

• Processo FAPESP: 10/16795-4 - Rastreamento de biomarcadores e do Papilomavírus humano em raspado celular de canal anal: correlação com infecção concomitante em colo uterino, cavidade oral e orofaringe
• Beneficiário: Adhemar Longatto Filho
• Modalidade de apoio: Auxílio à Pesquisa - Regular