New glycosylated conjugate copolymer N-acetyl-beta-D-glucosaminyl-pluronic: Synthesis, self-assembly and biological assays

Autor(es):Mostrar menos -

Allievi Frizon, Tiago Elias ^[1] ; Serafini Micheletto, Yasmine Miguel ^[1] ; Westrup, Jose Luiz ^[1] ; Silva Wakabayashi, Priscila Sayoko ^[1] ; Serafim, Francieli Rocha ^[1] ; Damiani, Adriani Paganini ^[2] ; Longaretti, Luiza Martins ^[2] ; de Andrade, Vanessa Moraes ^[2] ; Giacomelli, Fernando Carlos ^[3] ; Fort, Sebastien ^[4] ; Dal Bo, Alexandre Goncalves ^{[1, 4]}

Afiliação do(s) autor(es)

^[1] Univ Extremo Sul Catarinense, Lab Processamento Polimeros Avancados LAPPA, BR-88806000 Criciuma, SC - Brazil

^[2] Univ Extremo Sul Catarinense, PPGCS, Lab Biol Celular & Mol, BR-88806000 Criciuma, SC - Brazil

^[3] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210170 Santo Andre, SP - Brazil

^[4] Univ Grenoble Alpes, CERMAV, F-38000 Grenoble - France

• Tipo de documento: Artigo Científico
• Fonte: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 133, p. 323-330, SEP 1 2015.
• Citações Web of Science: 2

Resumo

This work describes the synthesis of a new glycosylated conjugate copolymer, GlcNAc-PEO75-PPO30-PEO75-GlcNAc (GlcNAc-PluronicF68-GIcNAc), using click chemistry from Pluronic (R) F68 and propargyl-2-N-acetamido-2-deoxy-beta-D-glucopyranoside. Micelles were prepared by the self-assembly of GlcNAc-PluronicF68-GlcNAc in phosphate-buffered solution. The critical micelle concentration was determined by fluorescence spectroscopy, and the value was found to be equal to 5.8 mg mL(-1). The Gibbs free energy (Delta G) of micellization is negative, indicating that the organization of amphiphiles is governed by the hydrophobic effects in an entropy-driven process. The scattering characterization of GlcNAc-PluronicF68-GlcNAc micelles showed a hydrodynamic radius of 8.7 nm and negative zeta potential (-21.0 +/- 0.9 mV). The TEM image evidences the spherical shape of the objects self-assemble into highly regular micelles having a mean diameter of 10 nm. The SAXS profile confirmed the spherical shape of the assemblies comprising a swollen PPO core (R-core = 2.25 nm) stabilized by PEO chains following Gaussian statistics. The results of the comet assay showed that the GlcNAc-PluronicF68-GlcNAc micelles were not genotoxic, and the cell viability test was higher than 97% for all concentrations, demonstrating that GlcNAc-PluronicF68-GlcNAc is not toxic. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 12/14087-8 - Desenvolvimento de sistemas poliméricos nanoestruturados com tamanho controlado e sua interação com ambientes biológicos
• Beneficiário: Fernando Carlos Giacomelli
• Linha de fomento: Auxílio à Pesquisa - Regular