Reduced graphene oxide-Sb2O5 hybrid nanomaterial for the design of a laccase-based amperometric biosensor for estriol

Autor(es)

Cincotto, Fernando H. ^{[1, 2]} ; Canevari, Thiago C. ^[3] ; Machado, Sergio A. S. ^[1] ; Sanchez, Alfredo ^[2] ; Ruiz Barrio, Maria Asuncion ^[2] ; Villalonga, Reynaldo ^[2] ; Pingarron, Jose M. ^[2]

Afiliação do(s) autor(es)

^[1] State Univ Sao Paulo, Inst Chem, BR-13560970 Sao Carlos, SP - Brazil

^[2] Univ Complutense Madrid, Fac Chem, Dept Analyt Chem, E-28040 Madrid - Spain

^[3] Mackenzie Presbiterian Univ, Sch Engn, BR-01302907 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Electrochimica Acta; v. 174, p. 332-339, AUG 20 2015.
• Citações Web of Science: 22

Resumo

A novel reduced graphene oxide/Sb2O5 hybrid nanomaterial was prepared by a one-pot reaction process. The nanomaterial was characterized by different spectroscopic, microscopic and electrochemical techniques, demonstrating that the graphene sheets were doped with a Sb2O5 thin film. A glassy carbon electrode coated with the hybrid material was further employed as support for the covalent immobilization of laccase to develop an electrochemical biosensor for estriol. The enzyme biosensor showed high sensitivity (275 mA/M) and fast analytical response (4 s) for the hormone, with a limit of detection of 11 nM in the range of 25 nM to 1.03 p,M. The biosensor showed high selectivity for the analytical detection of estriol. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 14/02457-0 - Preparação de nanomateriais híbridos avançados baseados em grafeno funcionalizados com polipirrol e nanopartículas de antimônio para a detecção bioeletroquimica mediada por lacase de compostos interferentes endócrinos
• Beneficiário: Fernando Henrique Cincotto
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 11/23047-7 - Desenvolvimento de biosensor eletroquímico utilizando sílica mesoporosa contendo nanotubos de carbono obtida pelo processo sol-gel. Determinação eletroquímica de pesticidas
• Beneficiário: Thiago da Cruz Canevari
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado