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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Antibacterial Properties Associated with Chitosan Nanoparticle Treatment on Root Dentin and 2 Types of Endodontic Sealers

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Autor(es):
del Carpio-Perochena, Aldo [1] ; Kishen, Anil [2] ; Shrestha, Annie [2] ; Bramante, Clovis Monteiro [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Dent Endodont & Dent Mat, Bauru Dent Sch, Sao Paulo - Brazil
[2] Univ Toronto, Fac Dent, Discipline Endodont, Toronto, ON - Canada
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF ENDODONTICS; v. 41, n. 8, p. 1353-1358, AUG 2015.
Citações Web of Science: 22
Resumo

Introduction: The aim of this study was to evaluate the efficacy of carboxymethyl chitosan (CMCS) and chitosan nanoparticles (CNps) to inactivate bacteria and prevent biofilm formation at sealer-dentin interfaces. Methods: The study was divided into 3 stages:.first stage, the experiment was conducted to analyze the antibacterial properties of CMCS in different formulations against biofilms; second stage, direct-contact and membranerestricted methods were used to evaluate the antibacterial properties of an epoxy resin (ThermaSeal Plus; Dentsply Tulsa Dental, Tulsa, OK) and calcium silicate (MTA Fillapex; Angelus SA, Londrina, PR, Brazil) based-sealers with or without CNps; and third stage, biofilm formation at the sealer dentin interfaces of root dentin treated with CMCS and filled with gutta.-percha and CNp incorporated sealer were analyzed after 1- and 4-week aging periods. The samples were treated and filled as follows: (1) distilled water: unaltered sealer (control group), (2) CMCS: sealer+CNps.(CMCS group), and (3) CMCS/rosd bengal: sealer+CNps (CMCS/RB group). Enterococcus faecalis was used to infect all the samples. Microbiological and microscopic analyses were used to assess the antibacterial characteristics. Results: CMCS-based treatments effectively killed bacteria adherent on root dentin (P<.05). The addition of CNps to ThermaSeal enhanced its antibacterial ability by direct-contact and membrane-restricted tests (P<.05). The CNp incorporation significantly increased the antibacterial efficacy of root canal sealers even after a 4-week aging time (P<.05). Conclusions: This study highlighted the ability of CMCS to disinfect root canal dentin and inhibit bacterial adhesion. CNps in root canal sealers are capable of maintaining their antibacterial activity even after prolonged aging. (AU)

Processo FAPESP: 11/08184-8 - Efeito das nanopartículas de quitosana e da variação da concentração, temperatura e ph do hipoclorito de sódio sobre biofilmes orais formados in situ.
Beneficiário:Aldo Enrique Del Carpio Perochena
Modalidade de apoio: Bolsas no Brasil - Doutorado
Processo FAPESP: 13/09239-6 - Propriedades antibacterianas e antibiofílmicas associadas às nanopartículas de quitosana sobre dentina radicular e cimentos endodônticos
Beneficiário:Aldo Enrique Del Carpio Perochena
Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado