Antibacterial Properties Associated with Chitosan Nanoparticle Treatment on Root Dentin and 2 Types of Endodontic Sealers

Author(s)

del Carpio-Perochena, Aldo ^[1] ; Kishen, Anil ^[2] ; Shrestha, Annie ^[2] ; Bramante, Clovis Monteiro ^[1]

Affiliation

^[1] Univ Sao Paulo, Dept Dent Endodont & Dent Mat, Bauru Dent Sch, Sao Paulo - Brazil

^[2] Univ Toronto, Fac Dent, Discipline Endodont, Toronto, ON - Canada

• Document type: Journal article
• Source: JOURNAL OF ENDODONTICS; v. 41, n. 8, p. 1353-1358, AUG 2015.
• Web of Science Citations: 22

Abstract

Introduction: The aim of this study was to evaluate the efficacy of carboxymethyl chitosan (CMCS) and chitosan nanoparticles (CNps) to inactivate bacteria and prevent biofilm formation at sealer-dentin interfaces. Methods: The study was divided into 3 stages:.first stage, the experiment was conducted to analyze the antibacterial properties of CMCS in different formulations against biofilms; second stage, direct-contact and membranerestricted methods were used to evaluate the antibacterial properties of an epoxy resin (ThermaSeal Plus; Dentsply Tulsa Dental, Tulsa, OK) and calcium silicate (MTA Fillapex; Angelus SA, Londrina, PR, Brazil) based-sealers with or without CNps; and third stage, biofilm formation at the sealer dentin interfaces of root dentin treated with CMCS and filled with gutta.-percha and CNp incorporated sealer were analyzed after 1- and 4-week aging periods. The samples were treated and filled as follows: (1) distilled water: unaltered sealer (control group), (2) CMCS: sealer+CNps.(CMCS group), and (3) CMCS/rosd bengal: sealer+CNps (CMCS/RB group). Enterococcus faecalis was used to infect all the samples. Microbiological and microscopic analyses were used to assess the antibacterial characteristics. Results: CMCS-based treatments effectively killed bacteria adherent on root dentin (P<.05). The addition of CNps to ThermaSeal enhanced its antibacterial ability by direct-contact and membrane-restricted tests (P<.05). The CNp incorporation significantly increased the antibacterial efficacy of root canal sealers even after a 4-week aging time (P<.05). Conclusions: This study highlighted the ability of CMCS to disinfect root canal dentin and inhibit bacterial adhesion. CNps in root canal sealers are capable of maintaining their antibacterial activity even after prolonged aging. (AU)

• FAPESP's process: 11/08184-8 - EFFECT OF CHITOSAN NANOPARTICLES AND THE SODIUM HYPOCHLORITE IN DIFFERENT CONCENTRATION, TEMPERATURE AND PH ON ORAL BIOFILMS FORMED IN SITU
• Grantee: Aldo Enrique Del Carpio Perochena
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/09239-6 - Antibacterial and antibiofilm properties associated with chitosan nanoparticles on root dentin and endodontic sealers
• Grantee: Aldo Enrique Del Carpio Perochena
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate