Antibacterial and antibiofilm properties associated with chitosan nanoparticles on root dentin and endodontic sealers

• Grant number: 13/09239-6
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): March 05, 2014
• Effective date (End): September 04, 2014
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Clovis Monteiro Bramante
• Grantee: Aldo Enrique Del Carpio Perochena
• Supervisor: Anil Kishen
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• Research place: University of Toronto (U of T), Canada
• Associated to the scholarship: 11/08184-8 - EFFECT OF CHITOSAN NANOPARTICLES AND THE SODIUM HYPOCHLORITE IN DIFFERENT CONCENTRATION, TEMPERATURE AND PH ON ORAL BIOFILMS FORMED IN SITU, BP.DR

Abstract

Objective: the aim of this study will be evaluate the efficacy of chitosan nanoparticles to inactivate bacteria and prevent biofilm formation at the sealer-dentin interfaces. Material and methods: The study will be divided in three stages. First stage: the experiment will be conducted to analyse the antibacterial properties of carboxymethyl-chitosan (CMCS) in different formulations against biofilms. Second stage: direct-contact and membrane-restricted methods will be utilized to evaluate the antibacterial ability of an epoxy resin (ThermaSeal Plus) (TS) and calcium silicate (MTA Fillapex) (MTA) based-sealers associated or not with chitosan nanoparticles (CNp). Third stage: the biofilm formation at the sealer dentin interfaces of root canal dentin treated with CMCS and filled with gutta-percha and sealer incorporated with CNp will be analyzed after 1- and 4- weeks aging periods. The samples will be treated and filled as follow: (1) distilled water - unaltered sealer (control group), (2) CMCS - sealer+CNp (CMCS group), (3) CMCS/Rose Bengal - sealer+CNp (CMCS/RB group). Microbiological and microscopic analyses will be used to perform the study. E. faecalis will be used to infect the samples throughout the experiment. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DEL CARPIO-PEROCHENA, ALDO; KISHEN, ANIL; SHRESTHA, ANNIE; BRAMANTE, CLOVIS MONTEIRO. Antibacterial Properties Associated with Chitosan Nanoparticle Treatment on Root Dentin and 2 Types of Endodontic Sealers. JOURNAL OF ENDODONTICS, v. 41, n. 8, p. 1353-1358, AUG 2015. (11/08184-8, 13/09239-6)