Effect of tyrosol on adhesion of Candida albicans and Candida glabrata to acrylic surfaces

Autor(es)

Monteiro, Douglas Roberto ^[1] ; Feresin, Leonardo Perina ^[1] ; Arias, Lais Salomao ^[1] ; Ricardo Barao, Valentim Adelino ^[2] ; Barbosa, Debora Barros ^[3] ; Botazzo Delbem, Alberto Carlos ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Dept Pediat Dent & Publ Hlth, Aracatuba Dent Sch, BR-16015050 Aracatuba Sao Paulo - Brazil

^[2] Univ Estadual Campinas, UNICAMP, Piracicaba Dent Sch, Dept Prosthodont & Periodontol, BR-13414903 Piracicaba Sao Paulo - Brazil

^[3] Univ Estadual Paulista, UNESP, Aracatuba Dent Sch, Dept Dent Mat & Prosthodont, BR-16015050 Aracatuba Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Medical Mycology; v. 53, n. 7, p. 656-665, SEP 2015.
• Citações Web of Science: 15

Resumo

The prevention of adhesion of Candida cells to acrylic surfaces can be regarded as an alternative to prevent denture stomatitis. The use of quorum sensing molecules, such as tyrosol, could potentially interfere with the adhesion process. Therefore, the aim of this study was to assess the effect of tyrosol on adhesion of single and mixed cultures of Candida albicans and Candida glabrata to acrylic resin surfaces. Tyrosol was diluted in each yeast inoculum (10(7) cells/ml in artificial saliva) at 25, 50, 100, and 200 mM. Then, each dilution was added to wells of 24-well plates containing the acrylic specimens, and the plates were incubated at 37A degrees C for 2 h. After, the effect of tyrosol was determined by total biomass quantification, metabolic activity of the cells and colony-forming unit counting. Chlorhexidine gluconate (CHG) was used as a positive control. Data were analyzed using analysis of variance (ANOVA) and the Holm-Sidak post hoc test (alpha = 0.05). The results of total biomass quantification and metabolic activity revealed that the tyrosol promoted significant reductions (ranging from 22.32 to 86.16%) on single C. albicans and mixed cultures. Moreover, tyrosol at 200 mM and CHG significantly reduced (p < 0.05) the number of adhered cells to the acrylic surface for single and mixed cultures of both species, with reductions ranging from 1.74 to 3.64-log(10.) In conclusion, tyrosol has an inhibitory effect on Candida adhesion to acrylic resin, and further investigations are warranted to clarify its potential against Candida infections. (AU)

• Processo FAPESP: 13/17767-2 - Efeito do Tirosol na capacidade de adesão de células de Candida à superfície de uma resina acrílica
• Beneficiário: Leonardo Perina Feresin
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 13/10285-2 - Efeito do tirosol sobre fatores de virulência de Candida albicans, Candida glabrata e Streptococcus mutans
• Beneficiário: Alberto Carlos Botazzo Delbem
• Modalidade de apoio: Auxílio à Pesquisa - Regular