Three Phoma spp. synthesised novel silver nanoparticles that possess excellent antimicrobial efficacy

Autor(es)

Rai, Mahendra ^{[1, 2]} ; Ingle, Avinash P. ^[2] ; Gade, Aniket K. ^{[3, 2]} ; Teixeira Duarte, Marta Cristina ^[4] ; Duran, Nelson ^{[1, 5]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Chem, Biol Chem Lab, Campinas, SP - Brazil

^[2] SGB Amravati Univ, Dept Biotechnol, Nanobiotechnol Lab, Amravati 444602, Maharashtra - India

^[3] Utah State Univ, Dept Biol, Logan, UT 84322 - USA

^[4] Univ Estadual Campinas, Res Ctr Chem Biol & Agr, BR-13083970 Campinas, SP - Brazil

^[5] Lab Nanostruct Synth & Biol Interact NanoBioss UN, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: IET Nanobiotechnology; v. 9, n. 5, p. 280-287, OCT 2015.
• Citações Web of Science: 13

Resumo

The authors report extracellular mycosynthesis of silver nanoparticles (AgNPs) by Phoma capsulatum, Phoma putaminum and Phoma citri. The AgNPs thus synthesised were characterised by UV-visible spectrophotometer, Fourier transform infrared spectroscopy, Nanosight LM20 and transmission electron microscopy, which confirmed the synthesis of mostly spherical and polydisperse nanoparticles capped with proteins. The size of AgNPs was found in the range of 10-80 , 5-80 and 5-90 nm with an average size of 31.85, 25.43 and 23.29 nm by P. capsulatum, P. putaminum and P. citri, respectively. Further, potential antimicrobial activity was reported against Aspergillus niger, Candida albicans, Salmonella choleraesuis, Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli. The lowest minimal inhibitory concentration (MIC) (0.85 mu g/ml) was reported for AgNPs synthesised from P. citri against S. choleraesuis. However, AgNPs synthesised from P. capsulatum showed the highest MIC (10.62 mu g/ml) against S. choleraesuis, P. aeruginosa and E. coli (clinical isolate). The same MIC values (10.62 mu g/ml) were also reported against P. aeruginosa and both clinical and standard isolates of E. coli for AgNPs synthesised from P. citri. It was also observed that all the silver nanoparticles showed remarkable antifungal and antibacterial activity against these tested pathogens as compared with the commercially available antifungal and antibacterial agents. (AU)

• Processo FAPESP: 12/03731-3 - Biossíntese de nanopartículas de prata por seleção de espécies de Phoma e suas atividades contra micróbios multirresistentes
• Beneficiário: Nelson Eduardo Duran Caballero
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisador Visitante - Internacional