Three Phoma spp. synthesised novel silver nanoparticles that possess excellent antimicrobial efficacy

Author(s)

Rai, Mahendra ^{[1, 2]} ; Ingle, Avinash P. ^[2] ; Gade, Aniket K. ^{[3, 2]} ; Teixeira Duarte, Marta Cristina ^[4] ; Duran, Nelson ^{[1, 5]}

Affiliation

^[1] Univ Estadual Campinas, Inst Chem, Biol Chem Lab, Campinas, SP - Brazil

^[2] SGB Amravati Univ, Dept Biotechnol, Nanobiotechnol Lab, Amravati 444602, Maharashtra - India

^[3] Utah State Univ, Dept Biol, Logan, UT 84322 - USA

^[4] Univ Estadual Campinas, Res Ctr Chem Biol & Agr, BR-13083970 Campinas, SP - Brazil

^[5] Lab Nanostruct Synth & Biol Interact NanoBioss UN, Campinas, SP - Brazil

• Document type: Journal article
• Source: IET Nanobiotechnology; v. 9, n. 5, p. 280-287, OCT 2015.
• Web of Science Citations: 13

Abstract

The authors report extracellular mycosynthesis of silver nanoparticles (AgNPs) by Phoma capsulatum, Phoma putaminum and Phoma citri. The AgNPs thus synthesised were characterised by UV-visible spectrophotometer, Fourier transform infrared spectroscopy, Nanosight LM20 and transmission electron microscopy, which confirmed the synthesis of mostly spherical and polydisperse nanoparticles capped with proteins. The size of AgNPs was found in the range of 10-80 , 5-80 and 5-90 nm with an average size of 31.85, 25.43 and 23.29 nm by P. capsulatum, P. putaminum and P. citri, respectively. Further, potential antimicrobial activity was reported against Aspergillus niger, Candida albicans, Salmonella choleraesuis, Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli. The lowest minimal inhibitory concentration (MIC) (0.85 mu g/ml) was reported for AgNPs synthesised from P. citri against S. choleraesuis. However, AgNPs synthesised from P. capsulatum showed the highest MIC (10.62 mu g/ml) against S. choleraesuis, P. aeruginosa and E. coli (clinical isolate). The same MIC values (10.62 mu g/ml) were also reported against P. aeruginosa and both clinical and standard isolates of E. coli for AgNPs synthesised from P. citri. It was also observed that all the silver nanoparticles showed remarkable antifungal and antibacterial activity against these tested pathogens as compared with the commercially available antifungal and antibacterial agents. (AU)

• FAPESP's process: 12/03731-3 - Biosynthesis of silver nanoparticles by selected species of Phoma and their activity against multi-drug resistant microbes
• Grantee: Nelson Eduardo Duran Caballero
• Support Opportunities: Research Grants - Visiting Researcher Grant - International