The centrally projecting Edinger-Westphal nucleus-I: Efferents in the rat brain

Autor(es):Mostrar menos -

Dos Santos Junior, Edmilson D. ^[1] ; Da Silva, Andre V. ^{[2, 1, 3]} ; Da Silva, Kelly R. T. ^{[2, 1]} ; Haemmerle, Carlos A. S. ^[1] ; Batagello, Daniella S. ^{[4, 1]} ; Da Silva, Joelcimar M. ^[1] ; Lima, Leandro B. ^[5] ; Da Silva, Renata J. ^[1] ; Diniz, Giovanne B. ^[1] ; Sita, Luciane V. ^[1] ; Elias, Carol F. ^{[1, 6]} ; Bittencourt, Jackson C. ^{[4, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Anat, Lab Chem Neuroanat, ICB, BR-05508000 Sao Paulo, SP - Brazil

^[2] Sao Paulo State Univ, Inst Biosci, Dept Anat, BR-18618970 Botucatu, SP - Brazil

^[3] Univ Fed Mato Grosso do Sul, BR-79600080 Tres Lagoas, MS - Brazil

^[4] Univ Sao Paulo, Inst Psychol, Ctr Neurosci & Behav, BR-05508030 Sao Paulo, SP - Brazil

^[5] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508000 Sao Paulo, SP - Brazil

^[6] Univ Michigan, Dept Mol & Integrat Physiol, Dept Obstet & Gynecol, Ann Arbor, MI 48109 - USA

• Tipo de documento: Artigo Científico
• Fonte: Journal of Chemical Neuroanatomy; v. 68, p. 22-38, OCT 2015.
• Citações Web of Science: 13

Resumo

The oculomotor accessory nucleus, often referred to as the Edinger-Westphal nucleus {[}EW], was first identified in the 17th century. Although its most well known function is the control of pupil diameter, some controversy has arisen regarding the exact location of these preganglionic neurons. Currently, the EW is thought to consist of two different parts. The first part {[}termed the preganglionic EW-EWpg], which controls lens accommodation, choroidal blood flow and pupillary constriction, primarily consists of cholinergic cells that project to the ciliary ganglion. The second part {[}termed the centrally projecting EW-EWcp], which is involved in non-ocular functions such as feeding behavior, stress responses, addiction and pain, consists of peptidergic neurons that project to the brainstem, the spinal cord and prosencephalic regions. However, in the literature, we found few reports related to either ascending or descending projections from the EWcp that are compatible with its currently described functions. Therefore, the objective of the present study was to systematically investigate the ascending and descending projections of the EW in the rat brain. We injected the anterograde tracer biotinylated dextran amine into the EW or the retrograde tracer cholera toxin subunit B into multiple EW targets as controls. Additionally, we investigated the potential EW-mediated innervation of neuronal populations with known neurochemical signatures, such as melanin-concentrating hormone in the lateral hypothalamic area {[}LHA] and corticotropin-releasing factor in the central nucleus of the amygdala {[}CeM]. We observed anterogradely labeled fibers in the LHA, the reuniens thalamic nucleus, the oval part of the bed nucleus of the stria terminalis, the medial part of the central nucleus of the amygdala, and the zona incerta. We confirmed our EW-LHA and EW-CeM connections using retrograde tracers. We also observed moderate EW-mediated innervation of the paraventricular nucleus of the hypothalamus and the posterior hypothalamus. Our findings provide anatomical bases for previously unrecognized roles of the EW in the modulation of several physiologic systems. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 07/56975-9 - Estudo das aferências imunorreativas ao hormônio concentrador de melanina (MCH) do núcleo accumbens, no rato Long Evans
• Beneficiário: Carlos Alexandre dos Santos Haemmerle
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 02/11237-7 - Caracterizacao neuroquimica e estudo das projecoes do nucleo acessorio do oculomotor.
• Beneficiário: Edmilson Duarte dos Santos Junior
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 04/13849-5 - Circuitos peptidérgicos envolvidos na organização do comportamento alimentar
• Beneficiário: Jackson Cioni Bittencourt
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 08/02771-6 - Estudo hodológico, neuroquímico e comportamental das aferências ao primeiro núcleo do circuito neural do reflexo auditivo de sobressalto: núcleo da raiz coclear
• Beneficiário: José de Anchieta de Castro e Horta Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 11/09816-8 - Substrato anatômico para o papel neuroendócrino da área incerto-hipotalâmica
• Beneficiário: Luciane Valéria Sita
• Modalidade de apoio: Auxílio à Pesquisa - Regular