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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Backbone and side chain NMR assignments of Geobacillus stearothermophilus ZapA allow identification of residues that mediate the interaction of ZapA with FtsZ

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Autor(es):
Nogueira, Maria Luiza C. [1] ; Sforca, Mauricio Luis [1] ; Chin, Yanni K. -Y. [2] ; Mobli, Mehdi [3] ; Handler, Aaron [4] ; Gorbatyuk, Vitaliy Y. [5] ; Robson, Scott A. [6] ; King, Glenn F. [2] ; Gueiros-Filho, Frederico J. [7] ; de Mattos Zeri, Ana Carolina [1]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Brazilian Biosci Natl Lab LNBio CNPEM, Campinas - Brazil
[2] Univ Queensland, Inst Mol Biosci, Brisbane, Qld - Australia
[3] Univ Queensland, Ctr Adv Imaging, Brisbane, Qld - Australia
[4] Genzyme Sanofi US, Bioanalyt Sci DSAR Clin Lab Sci, Framingham, MA - USA
[5] Univ Connecticut, BioNMR Facil, Biotechnol Bioserv Ctr, Mansfield, CT - USA
[6] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 - USA
[7] Univ Sao Paulo, Dept Bioquim, IQ, Sao Paulo - Brazil
Número total de Afiliações: 7
Tipo de documento: Artigo Científico
Fonte: BIOMOLECULAR NMR ASSIGNMENTS; v. 9, n. 2, p. 387-391, OCT 2015.
Citações Web of Science: 1
Resumo

Bacterial division begins with the formation of a contractile protein ring at midcell, which constricts the bacterial envelope to generate two daughter cells. The central component of the division ring is FtsZ, a tubulin-like protein capable of self-assembling into filaments which further associate into a higher order structure known as the Z ring. Proteins that bind to FtsZ play a crucial role in the formation and regulation of the Z ring. One such protein is ZapA, a widely conserved 21 kDa homodimeric protein that associates with FtsZ filaments and promotes their bundling. Although ZapA was discovered more than a decade ago, the structural details of its interaction with FtsZ remain unknown. In this work, backbone and side chain NMR assignments for the Geobacillus stearothermophilus ZapA homodimer are described. We titrated FtsZ into (NH)-N-15-H-2-ZapA and mapped ZapA residues whose resonances are perturbed upon FtsZ binding. This information provides a structural understanding of the interaction between FtsZ and ZapA. (AU)

Processo FAPESP: 10/51866-0 - SMolBNet 2.0: combinando genética e RMN para dissecar interações proteína-proteína fundamentais para o funcionamento do complexo de divisão bacteriana
Beneficiário:Frederico José Gueiros Filho
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 12/24916-1 - Estudos estruturais da proteína ZapA de B. stearothermophilus
Beneficiário:Maria Luiza Caldas Nogueira
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
Processo FAPESP: 12/15123-8 - Caracterização funcional e estrutural da proteína ZapA de Bacillus
Beneficiário:Maria Luiza Caldas Nogueira
Linha de fomento: Bolsas no Brasil - Doutorado