Ultraconserved Elements Sequencing as a Low-Cost Source of Complete Mitochondrial Genomes and Microsatellite Markers in Non-Model Amniotes

Autor(es)

do Amaral, Fabio Raposo ^[1] ; Neves, Leandro G. ^[2] ; Resende, Jr., Marcio F. R. ^[2] ; Mobili, Flavia ^[1] ; Miyaki, Cristina Y. ^[3] ; Pellegrino, Katia C. M. ^[1] ; Biondo, Cibele ^[4]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Lab Genet Evolut, BR-09972270 Diadema, SP - Brazil

^[2] RAPiD Genom LLC, Gainesville, FL 32601 - USA

^[3] Univ Sao Paulo, Dept Genet & Biol Evolut, BR-05508090 Sao Paulo, SP - Brazil

^[4] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09606070 Sao Bernardo Do Campo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 10, n. 9 SEP 17 2015.
• Citações Web of Science: 7

Resumo

Sequence capture of ultraconserved elements (UCEs) associated with massively parallel sequencing has become a common source of nuclear data for studies of animal systematics and phylogeography. However, mitochondrial and microsatellite variation are still commonly used in various kinds of molecular studies, and probably will complement genomic data in years to come. Here we show that besides providing abundant genomic data, UCE sequencing is an excellent source of both sequences for microsatellite loci design and complete mitochondrial genomes with high sequencing depth. Identification of dozens of microsatellite loci and assembly of complete mitogenomes is exemplified here using three species of Poospiza warbling finches from southern and southeastern Brazil. This strategy opens exciting opportunities to simultaneously analyze genome-wide nuclear datasets and traditionally used mtDNA and microsatellite markers in non-model amniotes at no additional cost. (AU)

• Processo FAPESP: 13/50297-0 - Dimensions US-BIOTA São Paulo: integrando disciplinas para a predição da biodiversidade da Floresta Atlântica no Brasil
• Beneficiário: Cristina Yumi Miyaki
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 11/23155-4 - Filogeografia multilocus comparada de três espécies de Poospiza (Aves, Passeriformes): explorando a história da Mata Atlântica montana
• Beneficiário: Fábio Sarubbi Raposo do Amaral
• Modalidade de apoio: Bolsas no Brasil - Programa BIOTA - Jovens Pesquisadores