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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Ultraconserved Elements Sequencing as a Low-Cost Source of Complete Mitochondrial Genomes and Microsatellite Markers in Non-Model Amniotes

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Author(s):
do Amaral, Fabio Raposo [1] ; Neves, Leandro G. [2] ; Resende, Jr., Marcio F. R. [2] ; Mobili, Flavia [1] ; Miyaki, Cristina Y. [3] ; Pellegrino, Katia C. M. [1] ; Biondo, Cibele [4]
Total Authors: 7
Affiliation:
[1] Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Lab Genet Evolut, BR-09972270 Diadema, SP - Brazil
[2] RAPiD Genom LLC, Gainesville, FL 32601 - USA
[3] Univ Sao Paulo, Dept Genet & Biol Evolut, BR-05508090 Sao Paulo, SP - Brazil
[4] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09606070 Sao Bernardo Do Campo, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: PLoS One; v. 10, n. 9 SEP 17 2015.
Web of Science Citations: 7
Abstract

Sequence capture of ultraconserved elements (UCEs) associated with massively parallel sequencing has become a common source of nuclear data for studies of animal systematics and phylogeography. However, mitochondrial and microsatellite variation are still commonly used in various kinds of molecular studies, and probably will complement genomic data in years to come. Here we show that besides providing abundant genomic data, UCE sequencing is an excellent source of both sequences for microsatellite loci design and complete mitochondrial genomes with high sequencing depth. Identification of dozens of microsatellite loci and assembly of complete mitogenomes is exemplified here using three species of Poospiza warbling finches from southern and southeastern Brazil. This strategy opens exciting opportunities to simultaneously analyze genome-wide nuclear datasets and traditionally used mtDNA and microsatellite markers in non-model amniotes at no additional cost. (AU)

FAPESP's process: 13/50297-0 - Dimensions US-BIOTA São Paulo: a multidisciplinary framework for biodiversity prediction in the Brazilian Atlantic forest hotspot
Grantee:Cristina Yumi Miyaki
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 11/23155-4 - Comparative multilocus phylogeography of three species of Poospiza (Aves, Passeriformes): exploring the history of the montane Atlantic Forest
Grantee:Fábio Sarubbi Raposo do Amaral
Support Opportunities: Scholarships in Brazil - BIOTA - Young Researchers