Transcriptome sequencing and microsatellites characterization in pirapitinga Piaractus brachypomus for analysis of genetic variability

• Grant number: 14/05732-2
• Support type: Scholarships in Brazil - Master
• Effective date (Start): June 01, 2014
• Effective date (End): February 29, 2016
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Diogo Teruo Hashimoto
• Grantee: Paulo Henrique Jorge
• Home Institution: Centro de Aquicultura (CAUNESP). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The pirapitinga, Piaractus brachypomus (Characiformes, Serrasalmidae), is a fish of occurrence in the Amazon Basin and one of the major native species used for aquaculture production. However, genetic studies focused to this species is practically absent, particularly on genetic variability of cultivated or wild stocks. In this case, the limiting factor to advance these studies is the lack of available molecular markers for P. brachypomus, considered a non-model organism in Aquaculture. Currently, Next-Generation Sequencing (NGS) technologies are causing a revolution in Molecular Biology as they have enabled the discovery of millions of SNPs and microsatellite markers in non-model organisms. This project aims to conduct the transcriptome sequencing of P. brachypomus by NGS and then characterize a set of microsatellite markers for this species. Finally, with the establishment of microsatellites, we intend to analyze broodstocks of pirapitinga cultivated in Brazilian fish farms, in order to identify the genetic profile of these animals and improve the genetic management of production systems. The characterization of molecular markers in P. brachypomus and genetic studies focusing on better knowledge of the genome of cultured broodstocks are considered essential for Aquaculture, especially to provide subsidies for the development of management and breeding programs of this species. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

JORGE, PAULO H.; MASTROCHIRICO-FILHO, VITO A.; HATA, MILENE E.; MENDES, NATALIA J.; ARIEDE, RAQUEL B.; DE FREITAS, MILENA VIEIRA; VERA, MANUEL; PORTO-FORESTI, FABIO; HASHIMOTO, DIOGO T. Genetic Characterization of the Fish Piaractus brachypomus by Microsatellites Derived from Transcriptome Sequencing. FRONTIERS IN GENETICS, v. 9, FEB 22 2018. Web of Science Citations: 4.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

JORGE, Paulo Henrique. Sequenciamento do transcriptoma e caracterização de microssatélites na pirapitinga Piaractus brachypomus para análises de variabilidade genética. 2016. Master's Dissertation - Universidade Estadual Paulista "Júlio de Mesquita Filho" Instituto de Biociências (Campus de Botucatu)..