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Integration of genomic methods for genotypes selection of pacu (Piaractus mesopotamicus) resistant to Aeromonas hydrophila bacteria

Grant number: 16/18294-9
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): December 01, 2016
Effective date (End): December 31, 2019
Field of knowledge:Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
Principal Investigator:Diogo Teruo Hashimoto
Grantee:Vito Antonio Mastrochirico Filho
Host Institution: Centro de Aquicultura (CAUNESP). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
Associated scholarship(s):17/26900-9 - RNA-seq and RAD-seq techniques for genetic characterization of pacu (Piaractus mesopotamicus) resistant to the bacteria Aeromonas hydrophila, BE.EP.DR


Selective breeding applied to aquaculture is a strategic research area for Brazil, in view the unavailability of genetically improved native species. This study aims to characterize genetically pacu (Piaractus mesopotamicus) resistant to the Aeromonas hydrophila bacteria. The specific objectives are: 1) characterization of immune system genes, by RNA-seq technique, of susceptible and resistant individuals challenged with A. hydrophila infection; 2) characterization of QTL (Quantitative Trait Locus) associated with resistance to A. hydrophila, by sequencing / genotyping of SNPs (ddRAD-seq, double-digest restriction- site-associated DNA sequencing). In relation to RNA-seq experiment, after inoculation of A. hydrophila, analyses will be performed at the following times: T1 (early death of animals), fishes that show signs of infection, considered susceptibles; T2 (after mortality plateau), individuals considered resistant to A. hydrophila challenge; and both T1 and T2, control treatment individuals inoculated with saline solution. Bioinformatic analyses will be performed from de novo assembly of pacu transcriptome, comparing T1 x Control, T2 x Control and T1 x T2. Afterwards, sequencing / genotyping will be performed by ddRAD-seq technique (restriction enzymes SphI and MluCI), in order to realize molecular analysis of 10 pacu families challenged with A. hydrophila, which will allow the search for QTL and provide support for marker-assisted selection. The potential impact of this study is to make feasible technologies for production of improved fishes for the aquaculture industry.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MASTROCHIRICO-FILHO, VITO A.; ARIEDE, RAQUEL B.; FREITAS, V, MILENA; LIRA, LIESCHEN V. G.; AGUDELO, JOHN F. G.; PILARSKI, FABIANA; REIS NETO, V, RAFAEL; YANEZ, JOSE M.; HASHIMOTO, DIOGO T.. Genetic parameters for resistance to Aeromonas hydrophila in the Neotropical fish pacu (Piaractus mesopotamicus). Aquaculture, v. 513, . (16/18294-9, 16/21011-9)
MASTROCHIRICO-FILHO, VITO A.; BORGES, CAROLINA H. S.; FREITAS, MILENA V.; ARIEDE, RAQUEL B.; PILARSKI, FABIANA; UTSUNOMIA, RICARDO; CARVALHEIRO, ROBERTO; GUTIERREZ, ALEJANDRO P.; PENALOZA, CAROLINA; YANEZ, JOSE M.; et al. Development of a SNP linkage map and genome-wide association study for resistance to Aeromonas hydrophila in pacu (Piaractus mesopotamicus). BMC Genomics, v. 21, n. 1, . (18/08416-5, 16/21011-9, 17/26900-9, 16/18294-9)
MASTROCHIRICO-FILHO, VITO A.; ARIEDE, RAQUEL B.; FREITAS, V, MILENA; BORGES, CAROLINA H. S.; LIRA, LIESCHEN V. G.; MENDES, NATALIA J.; AGUDELO, JOHN F. G.; CACERES, PABLO; BERROCAL, MILTHON H. M.; SUCERQUIA, GUSTAVO A. L.; et al. Development of a multi-species SNP array for serrasalmid fish Colossoma macropomum and Piaractus mesopotamicus. SCIENTIFIC REPORTS, v. 11, n. 1, . (16/21011-9, 17/26900-9, 20/11049-4, 16/18294-9, 18/08416-5, 17/19717-3, 19/10662-7, 19/08972-8)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MASTROCHIRICO FILHO, Vito Antonio. Integration of genomic methods for genotypes selection of pacu (Piaractus mesopotamicus) resistant to Aeromonas hydrophila bacteria. 2020. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.

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