Comparison of selected methods for recovery of Giardia spp. cysts and Cryptosporidium spp. oocysts in wastewater

Autor(es)

Medeiros, Raphael Correa ^[1] ; Daniel, Luiz Antonio ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Santa Maria, Dept Agron & Environm Sci, BR-98400000 Frederico Westphalen, RS - Brazil

^[2] Univ Sao Paulo, Sao Carlos Sch Engn, Hydraul & Sanitat Dept, BR-13566590 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF WATER AND HEALTH; v. 13, n. 3, p. 811-818, 2015.
• Citações Web of Science: 3

Resumo

More precise methods are needed to recover Giardia and Cryptosporidium (oo)cysts from wastewater in order to advance research related to their inactivation, removal, quantification, and species differentiation. This study applied different methods to recover the maximum number of (oo) cysts from wastewater samples using ColorSeed (R). Immunomagnetic separation assisted in capturing oocysts mainly in samples with medium and low turbidity. A triple centrifugation method reached recovery rates of 85% and 20%, for Giardia cysts and Cryptosporidium oocysts, respectively, in raw wastewater, and 62.5 and 17.5% in secondary-treated effluent. For low turbidity-treated effluent, membrane filtration reached 67.5% recovery for Giardia cysts and 22.5% for Cryptosporidium oocysts. Simple, quick and low-cost methods do not involve much handling of the samples and could be useful, particularly in developing countries. (AU)

• Processo FAPESP: 12/50522-0 - Contaminação ambiental pelos protozoários Giardia spp. e Cryptosporidium spp. e por Ascaris suum: desafios de detecção, remoção e inativação das formas infectantes
• Beneficiário: Regina Maura Bueno Franco
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 10/07902-1 - Avaliação do comportamento dos protozoários Giardia spp. e Cryptosporidium spp. e de microrganismos indicadores frente ao tratamento de esgoto e após o lançamento no corpo receptor.
• Beneficiário: Raphael Corrêa Medeiros
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/08653-8 - Remoção e inativação de microrganismos indicadores, cistos de Giardia spp. e de oocistos de Cryptosporidium spp. em sistemas de tratamento de esgoto sanitário
• Beneficiário: Luiz Antonio Daniel
• Modalidade de apoio: Auxílio à Pesquisa - Regular