Determination of the Mercury Fraction Linked to Protein of Muscle and Liver Tissue of Tucunar, (Cichla spp.) from the Amazon Region of Brazil

Autor(es)

Vieira, Jose C. S. ^[1] ; Cavecci, Bruna ^[1] ; Queiroz, Joao V. ^[2] ; Braga, Camila P. ^[1] ; Padilha, Cilene C. F. ^[1] ; Leite, Aline L. ^[3] ; Figueiredo, Wllyane S. ^[4] ; Buzalaf, Marilia A. R. ^[3] ; Zara, Luiz F. ^[4] ; Padilha, Pedro M. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, UNESP, Biosci Inst, Dept Chem & Biochem, BR-18618970 Botucatu, SP - Brazil

^[2] Sao Paulo State Univ, Coll Vet & Anim Sci, BR-18618970 Botucatu, SP - Brazil

^[3] Univ Sao Paulo, Dept Biol Sci, Bauru, SP - Brazil

^[4] Univ Brasilia, Coll Planaltina, Brasilia, DF - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Archives of Environmental Contamination and Toxicology; v. 69, n. 4, p. 422-430, NOV 2015.
• Citações Web of Science: 8

Resumo

This study used metalloproteomic techniques to characterize mercury (Hg)-bound proteins in the muscle and liver tissue of Tucunar, (Cichla spp.) collected at the Jirau Hydroelectric Power Plant in Madeira River Basin, Brazil. The proteome of the muscle and liver tissue was obtained after two steps of fractional precipitation and separating the proteins by 2-D polyacrylamide gel electrophoresis. Hg was identified and quantified in the protein spots by graphite furnace atomic absorption spectrometry after acid mineralization in an ultrasound bath. Hg with a molecular weight < 20 kDa and a concentration between 13.30 and 33.40 mg g(-1) was found in the protein spots. These protein spots were characterized by electrospray ionization tandem mass spectrometry after trypsin digestion. From a total of 12 analyzed spots, seven proteins showing Hg biomarker characteristics were identified: parvalbumin and its isoforms, ubiquitin-40S ribosomal protein S27a, zinc (Zn) finger and BTB domain-containing protein 24, and dual-specificity protein phosphatase 22-B. (AU)

• Processo FAPESP: 12/24035-5 - Estudo metaloproteômico do mercúrio em amostras de tecido hepático de peixes coletados na área de influência do AHE Jirau - Bacia do Rio Madeira
• Beneficiário: José Cavalcante Souza Vieira
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 13/21297-1 - Metalotioneínas em peixes da região amazônica: estudo metalômico associado ao mercúrio
• Beneficiário: Pedro de Magalhães Padilha
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/51332-5 - Desenvolvimento de métodos analíticos para estudo metalômico do mercúrio em peixes coletados na área de influência do AHE Jirau Bacia do Rio Madeira
• Beneficiário: Pedro de Magalhães Padilha
• Modalidade de apoio: Auxílio à Pesquisa - Regular