Determination of the Mercury Fraction Linked to Protein of Muscle and Liver Tissue of Tucunar, (Cichla spp.) from the Amazon Region of Brazil

Author(s)

Vieira, Jose C. S. ^[1] ; Cavecci, Bruna ^[1] ; Queiroz, Joao V. ^[2] ; Braga, Camila P. ^[1] ; Padilha, Cilene C. F. ^[1] ; Leite, Aline L. ^[3] ; Figueiredo, Wllyane S. ^[4] ; Buzalaf, Marilia A. R. ^[3] ; Zara, Luiz F. ^[4] ; Padilha, Pedro M. ^[1]

Affiliation

^[1] Sao Paulo State Univ, UNESP, Biosci Inst, Dept Chem & Biochem, BR-18618970 Botucatu, SP - Brazil

^[2] Sao Paulo State Univ, Coll Vet & Anim Sci, BR-18618970 Botucatu, SP - Brazil

^[3] Univ Sao Paulo, Dept Biol Sci, Bauru, SP - Brazil

^[4] Univ Brasilia, Coll Planaltina, Brasilia, DF - Brazil

• Document type: Journal article
• Source: Archives of Environmental Contamination and Toxicology; v. 69, n. 4, p. 422-430, NOV 2015.
• Web of Science Citations: 8

Abstract

This study used metalloproteomic techniques to characterize mercury (Hg)-bound proteins in the muscle and liver tissue of Tucunar, (Cichla spp.) collected at the Jirau Hydroelectric Power Plant in Madeira River Basin, Brazil. The proteome of the muscle and liver tissue was obtained after two steps of fractional precipitation and separating the proteins by 2-D polyacrylamide gel electrophoresis. Hg was identified and quantified in the protein spots by graphite furnace atomic absorption spectrometry after acid mineralization in an ultrasound bath. Hg with a molecular weight < 20 kDa and a concentration between 13.30 and 33.40 mg g(-1) was found in the protein spots. These protein spots were characterized by electrospray ionization tandem mass spectrometry after trypsin digestion. From a total of 12 analyzed spots, seven proteins showing Hg biomarker characteristics were identified: parvalbumin and its isoforms, ubiquitin-40S ribosomal protein S27a, zinc (Zn) finger and BTB domain-containing protein 24, and dual-specificity protein phosphatase 22-B. (AU)

• FAPESP's process: 12/24035-5 - Mercury metalloproteomics studies in hepatic tissue samples of fish collected in the the AHE JIRAU - Madeira River Basin area of influence
• Grantee: José Cavalcante Souza Vieira
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 13/21297-1 - Metallothioneins in fish from Amazon Region: metallomic study associated to mercury
• Grantee: Pedro de Magalhães Padilha
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/51332-5 - Development of analytical methods for mercury metallomics studies in fish collected in the AHE JIRAU - Madeira River Basin area of influence
• Grantee: Pedro de Magalhães Padilha
• Support Opportunities: Regular Research Grants