Advanced search
Start date
Betweenand

Utilization of metalloproteomic strategies for metallothionein evaluation in liver tissue samples of fishes collected in the reservoir of Ahe Jirau - Madeira River Basin, RO, Brazil

Grant number: 14/02668-1
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): September 01, 2014
Effective date (End): March 31, 2017
Field of knowledge:Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Pedro de Magalhães Padilha
Grantee:José Cavalcante Souza Vieira
Home Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Fish is an important source of protein with high biological value, vitamins and minerals. However, this food is also a major route of human exposure to toxic contaminants such as methylmercury (MeHg). Thus, their accumulation in fish may predispose serious risks for human health. The high levels of mercury found in soil, sediments, fish and humans in the Brazilian Amazon have been studied extensively by the scientific community in recent decades, seeking to elucidate mechanisms of toxicity of mercury species. The mercury species bind preferentially to metallothioneins - proteins of molecular weight below 10 kDa, which are characterized by having sulfur-containing amino acid residues, such as the cysteine. This may mean that the bioavailable fraction of the mercury species in the different species of fish can be dependent on how they are related to proteins. View of the above, this proposal work seeks to optimize: procedures for 2D-PAGE electrophoresis for fractionation and identification of metallothionein responsible for the transport of mercury in fish samples collected at the reservoir from AHE JIRAU - Madeira River Basin; a method for determination of mercury by GFAAS or CVAFS in spots protein obtained; speciation methodologies of mercury in samples of muscle tissue and liver; characterization of metallothioneins mercury carriers by ESI-MS and finally the analysis of expression of these proteins to looking for a possible correlation with the incorporation of mercury in these fishes. (AU)

Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SOUZA VIEIRA, JOSE CAVALCANTE; DE OLIVEIRA, GRASIELI; BRAGA, CAMILA PEREIRA; FERNANDES, MILENI DA SILVA; DE MORAES, PAULA MARTIN; RABELO BUZALAF, MARILIA AFONSO; SILVA DE OLIVEIRA, LINCOLN CARLOS; PADILHA, PEDRO DE MAGALHAES. Parvalbumin and Ubiquitin as Potential Biomarkers of Mercury Contamination of Amazonian Brazilian Fish. BIOLOGICAL TRACE ELEMENT RESEARCH, v. 197, n. 2, p. 667-675, OCT 2020. Web of Science Citations: 1.
BITTARELLO, ALIS CORREIA; SOUZA VIEIRA, JOSE CAVALCANTE; BRAGA, CAMILA PEREIRA; BATAGLIOLI, IZABELA DA CUNHA; DE OLIVEIRA, GRASIELI; ROCHA, LEONE CAMPOS; ZARA, LUIZ FABRICIO; RABELO BUZALAF, MARILIA AFONSO; SILVA DE OLIVEIRA, LINCOLN CARLOS; ADAMEC, JIRI; PADILHA, PEDRO DE MAGALHAES. Metalloproteomic approach of mercury -binding proteins in liver and kidney tissues of Plagioscion squamosissimus (corvina) and Colossoma macropomum (tambaqui) from Amazon region: Possible identification of mercury contamination biomarkers. Science of The Total Environment, v. 711, APR 1 2020. Web of Science Citations: 0.
BITTARELLO, ALIS CORREIA; SOUZA VIEIRA, JOSE CAVALCANTE; BRAGA, CAMILA PEREIRA; DE PAULA ARAUJO, WELLINGTON LUIZ; BATAGLIOLI, IZABELA DA CUNHA; DA SILVA, JANAINA MACEDO; RABELO BUZALAF, MARILIA AFONSO; FLEURI, LUCIANA FRANCISCO; PADILHA, PEDRO DE MAGALHAES. Characterization of molecular biomarkers of mercury exposure to muscle tissue of Plagioscion squamosissimus and Colossoma macropomum from the Amazon region. Food Chemistry, v. 276, p. 247-254, MAR 15 2019. Web of Science Citations: 3.
SOUZA VIEIRA, JOSE CAVALCANTE; BRAGA, CAMILA PEREIRA; DE OLIVEIRA, GRASIELI; FEDERICI PADILHA, CILENE DO CARMO; DE MORAES, PAULA MARTIN; ZARA, LUIZ FABRICIO; LEITE, ALINE DE LIMA; RABELO BUZALAF, MARILIA AFONSO; PADILHA, PEDRO DE MAGALHAES. Mercury Exposure: Protein Biomarkers of Mercury Exposure in Jaraqui Fish from the Amazon Region. BIOLOGICAL TRACE ELEMENT RESEARCH, v. 183, n. 1, p. 164-171, MAY 2018. Web of Science Citations: 6.
SOUZA VIEIRA, JOSE CAVALCANTE; BRAGA, CAMILA PEREIRA; DE OLIVEIRA, GRASIELI; LEITE, ALINE DE LIMA; DE QUEIROZ, JOAO VITOR; CAVECCI, BRUNA; BITTARELLO, ALIS CORREIA; RABELO BUZALAF, MARILIA AFONSO; ZARA, LUIZ FABRICIO; PADILHA, PEDRO DE MAGALHAES. Identification of protein biomarkers of mercury toxicity in fish. ENVIRONMENTAL CHEMISTRY LETTERS, v. 15, n. 4, p. 717-724, DEC 2017. Web of Science Citations: 8.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.