Low-level laser irradiation modulates cell viability and creatine kinase activity in C2C12 muscle cells during the differentiation process

Autor(es)

Mesquita-Ferrari, Raquel Agnelli ^[1] ; Alves, Agnelo Neves ^[1] ; Cardoso, Vinicius de Oliveira ^[1] ; Artilheiro, Paola Pelegrineli ^[1] ; Bussadori, Sandra Kalil ^[1] ; Rocha, Lilia Alves ^[2] ; Nunes, Fabio Daumas ^[3] ; Santos Fernandes, Kristianne Porta ^[1]

Afiliação do(s) autor(es)

^[1] Univ Nove Julho UNINOVE, Postgrad Program Rehabil Sci & Biophoton Appl Hlt, BR-01504001 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Sch Dent, Dept Mol Pathol, BR-05508000 Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Sch Dent, Dept Oral Pathol, BR-05508000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 30, n. 8, p. 2209-2213, NOV 2015.
• Citações Web of Science: 7

Resumo

Low-level laser irradiation (LLLI) is increasingly used to treat musculoskeletal disorders, with satisfactory results described in the literature. Skeletal muscle satellite cells play a key role in muscle regeneration. The aim of the present study was to evaluate the effect of LLLI on cell viability, creatine kinase (CK) activity, and the expression of myogenic regulatory factors in C2C12 myoblasts during the differentiation process. C2C12 cells were cultured in Dulbecco's modified Eagle's medium (DMEM) containing 2 % horse serum and submitted to irradiation with GaAlAs diode laser (wavelength, 780 nm; output power, 10 mW; energy density, 5 J/cm(2)). Cell viability and the expression of myogenic regulatory factors were assessed 24, 48, and 72 h after irradiation by 3-(4,5-dimethylthiazol-2-yl)-2,5,-diphenyltetrazolium bromide (MTT) assay and quantitative real-time polymerase chain reaction (RT-qPCR), respectively. CK activity was analyzed at 24 and 72 h. An increase in cell viability was found in the laser group in comparison to the control group at all evaluation times. CK activity was significantly increased in the laser group at 72 h. Myogenin messenger RNA (mRNA) demonstrated a tendency toward an increase in the laser group, but the difference in comparison to the control group was non-significant. In conclusion, LLLI was able to modulate cell viability and CK activity in C2C12 myoblasts during the differentiation process. (AU)

• Processo FAPESP: 13/21540-3 - Efeito comparativo da terapia laser de baixa potência e do ultrassom da baixa intensidade sobre o fenótipo de fibras musculares, estresse oxidativo e proteínas envolvidas na regulação do reparo muscular
• Beneficiário: Agnelo Neves Alves
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/09191-5 - Avaliação dos efeitos do laser de baixa potência na proliferação e diferenciação de mioblastos C2C12
• Beneficiário: Raquel Agnelli Mesquita Ferrari
• Modalidade de apoio: Auxílio à Pesquisa - Regular