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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Temporal lobe epilepsy patients with severe hippocampal neuron loss but normal hippocampal volume: Extracellular matrix molecules are important for the maintenance of hippocampal volume

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Autor(es):
Peixoto-Santos, Jose Eduardo [1] ; Velasco, Tonicarlo Rodrigues [1] ; Galvis-Alonso, Orfa Yineth [2] ; Araujo, David [3] ; Kandratavicius, Ludmyla [1] ; Assirati, Joao Alberto [4] ; Carlotti, Carlos Gilberto [4] ; Scandiuzzi, Renata Caldo [1] ; dos Santos, Antonio Carlos [5] ; Leite, Joao Pereira [1]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Neurosci & Behav, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Sao Jose do Rio Preto Med Sch, Dept Mol Biol, Sao Jose Do Rio Preto, SP - Brazil
[3] McGill Univ, Montreal Neurol Inst, Montreal, PQ - Canada
[4] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Surg, BR-14049900 Ribeirao Preto, SP - Brazil
[5] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Internal Med, BR-14049900 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Epilepsia; v. 56, n. 10, p. 1562-1570, OCT 2015.
Citações Web of Science: 14
Resumo

ObjectiveHippocampal sclerosis is a common finding in patients with temporal lobe epilepsy (TLE), and magnetic resonance imaging (MRI) studies associate the reduction of hippocampal volume with the neuron loss seen on histologic evaluation. Astrogliosis and increased levels of chondroitin sulfate, a major component of brain extracellular matrix, are also seen in hippocampal sclerosis. Our aim was to evaluate the association between hippocampal volume and chondroitin sulfate, as well as neuronal and astroglial populations in the hippocampus of patients with TLE. MethodsPatients with drug-resistant TLE were subdivided, according to hippocampal volume measured by MRI, into two groups: hippocampal atrophy (HA) or normal volume (NV) cases. Hippocampi from TLE patients and age-matched controls were submitted to immunohistochemistry to evaluate neuronal population, astroglial population, and chondroitin sulfate expression with antibodies against neuron nuclei protein (NeuN), glial fibrillary acidic protein (GFAP), and chondroitin sulfate (CS-56) antigens, respectively. ResultsBoth TLE groups were clinically similar. NV cases had higher hippocampal volume, both ipsilateral and contralateral, when compared to HA. Compared to controls, NV and HA patients had reduced neuron density, and increased GFAP and CS-56 immunopositive area. There was no statistical difference between NV and HA groups in neuron density or immunopositive areas for GFAP and CS-56. Hippocampal volume correlated positively with neuron density in CA1 and prosubiculum, and with immunopositive areas for CS-56 in CA1, and negatively with immunopositive area for GFAP in CA1. Multiple linear regression analysis indicated that both neuron density and CS-56 immunopositive area in CA1 were statistically significant predictors of hippocampal volume. SignificanceOur findings indicate that neuron density and chondroitin sulfate immunopositive area in the CA1 subfield are crucial for the hippocampal volume, and that chondroitin sulfate is important for the maintenance of a normal hippocampal volume in some cases with severe neuron loss. (AU)

Processo FAPESP: 08/52657-5 - Estudo do padrão da gliose e da expressão das metalotioneínas I/II e da aquaporina-4 no hipocampo de pacientes com epilepsia do lobo temporal
Beneficiário:José Eduardo Peixoto Santos
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 10/51515-2 - Estudo das relações entre populações celulares, expressão de aquaporina-4 e sulfato de condroitina com o tempo de relaxamento e taxa de transferência de magnetização no hipocampo de pacientes com..
Beneficiário:José Eduardo Peixoto Santos
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 09/53447-7 - Mecanismos sinápticos e não-sinápticos das epilepsias refratárias e suas implicações na busca de novas estratégias terapêuticas: uma abordagem translacional
Beneficiário:João Pereira Leite
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 05/56447-7 - Pesquisa através de imagens por ressonância magnética de alto campo destinadas a estudos em humanos
Beneficiário:João Pereira Leite
Linha de fomento: Auxílio à Pesquisa - Programa CINAPCE - Temático