Biodegradation of pentachlorophenol by marine-derived fungus Trichoderma harziam CBMAI 1677 isolated from ascidian Didemnun ligulum

Autor(es)

Vacondio, Bruna ^[1] ; Birolli, Willian G. ^[2] ; Ferreira, Irlon M. ^[2] ; Seleghim, Mirna H. R. ^[1] ; Goncalves, Sarah ^[3] ; Vasconcellos, Suzan P. ^[4] ; Porto, Andre L. M. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Lab Microbiol & Microrganismos Aquat, Dept Ecol & Biol Evolut, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Sao Paulo, Lab Quim Organ & Biocatalise, Inst Quim Sao Carlos, BR-13563120 Sao Carlos, SP - Brazil

^[3] Univ Fed Sao Paulo, Dept Med, Disciplina Doencas Infecciosas & Parasitarias, BR-04024062 Sao Paulo, SP - Brazil

^[4] Univ Fed Sao Paulo, Div Microbiol Imunol & Parasitol, Dept Ciencias Biol, BR-09913030 Diadema, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY; v. 4, n. 2, p. 266-275, APR 2015.
• Citações Web of Science: 13

Resumo

In this study, the marine-derived fungi Aspergillus sydowii DL6A. Apergillus yersicolor DL5A, Cladosporium oxysporurn DL5G, Fasurizirti proliferatum DMA and Trichoderma harzianurn CBMAI 1677 isolated from marine ascidian Didemnun ligulum were evaluated according to their growth in the presence of pentachlorophenol (PCP). The colonies were assessed in 10, 20, 30, 40 and 50 mg L-1 of PCP in a solid culture medium (3% malt). The fungus T. harzianurn CBMAI 1677 showed the best growth at 50 mg L-1, which suggests its potential for biodegradation, therefore, this strain was selected for quantitative experiments in 3% malt liquid medium (initial concentration of 20 mg L-1 of PCP) using a validated method. After 7 d of incubation, PCP was not detected and an increasing concentration of pentachloroanisole (PCA) and 2,3,4,6-tetrachloroanisole (2,3,4,6-TeCA) was observed. In a second step, T harzianum CBMAI 1677 was employed in the biodegradation of PCA and 2,3,4,6-TeCA in a liquid medium It was observed that both PCA and 2,3,4,6-TeCA were also biodegraded. T harzianum CBMAI 1677 is a potential strain for bioremecliation studies since it was able of biodegrade not only PCP at 20 mg L-1, but also its main metabolites PCA and 2,3,4,6-TeCA. (C) 2015 Elsevier Ltd. All rights reserved (AU)

• Processo FAPESP: 12/19934-0 - Biodegradação de pesticidas por micro-organismos (fungos e bactérias)
• Beneficiário: Andre Luiz Meleiro Porto
• Modalidade de apoio: Auxílio à Pesquisa - Regular