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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

DNA barcode and evolutionary relationship within Laemolyta Cope 1872 (Characiformes: Anostomidae) through molecular analyses

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Autor(es):
Ramirez, Jorge L. [1] ; Galetti, Jr., Pedro M. [1]
Número total de Autores: 2
Afiliação do(s) autor(es):
[1] Univ Fed Sao Carlos, Dept Genet & Evolucao, Lab Biodiversidade Mol & Conservacao, BR-13565905 Sao Carlos, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Molecular Phylogenetics and Evolution; v. 93, p. 77-82, DEC 2015.
Citações Web of Science: 9
Resumo

The Laemolyta genus is a monophyletic group with five valid species. Phylogenetic relationships among the species of this genus are unknown. We analyzed four nominal Laemolyta species. The COI gene for all individuals was amplified and the genetic distances were estimated. We performed genetic distance analyses to determine the different MOTUs. Two mitochondrial (COI and CytB) and three nuclear (Myh6, RAG1 and RAG2) markers were amplified for one individual of each identified MOTU. Maximum Parsimony and Maximum Likelihood were conducted using concatenate alignment. In addition, multilocus Bayesian species tree was carried out. By using DNA barcode, we identified six different MOTUs. The COI inter-MOTU distances ranged from 0.92% to 5.76%. The normalized mean intra-MOTU distance was 0.13%. The DNA barcode was useful to diagnose all species. Two clades showing distinct color patterns were recovered in all molecular phylogenetic trees. Clade A joined fishes with no vertical bars (L. garmani, L. taeniata 1 and L taeniata 2) and clade B, fishes with vertical dark bars (L. fernandezi Araguaia, L. fernandezi Xingu, and L. proxima). The results were able to identify the cryptic biodiversity within the group and obtained the most complete Laemolyta phylogeny. (C) 2015 Elsevier Inc. All rights reserved. (AU)

Processo FAPESP: 11/21836-4 - Filogenia molecular dos Anostomidae e filogeografia das espécies com cromossomos sexuais ZZ/ZW do gênero Leporinus
Beneficiário:Jorge Luis Ramirez Malaver
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 10/52315-7 - Predadores de topo de cadeia alimentar
Beneficiário:Pedro Manoel Galetti Junior
Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Regular