Genomic analyses and expression evaluation of thaumatin-like gene family in the cacao fungal pathogen Moniliophthora perniciosa

Autor(es)

Franco, Sulamita de Freitas ^[1] ; Baroni, Renata Moro ^{[2, 1]} ; Carazzolle, Marcelo Falsarella ^[1] ; Pereira Lima Teixeira, Paulo Jose ^[1] ; Reis, Osvaldo ^[1] ; Guimardes Pereira, Goncalo Amarante ^[1] ; Costa Mondego, Jorge Mauricio ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Biol, Dept Genet Evolucao & Bioagentes, Lab Genom & Expressao, Campinas, SP - Brazil

^[2] Inst Agron Estado Sao Paulo, Ctr Pesquisa & Desenvolvimento Recursos Genet Veg, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Biochemical and Biophysical Research Communications; v. 466, n. 4, p. 629-636, OCT 30 2015.
• Citações Web of Science: 7

Resumo

Thaumatin-like proteins (TLPs) are found in diverse eukaryotes. Plant TLPs, known as Pathogenicity Related Protein (PR-5), are considered fungal inhibitors. However, genes encoding TLPs are frequently found in fungal genomes. In this work, we have identified that Moniliophthora perniciosa, a basidiomycete pathogen that causes the Witches' Broom Disease (WBD) of cacao, presents thirteen putative TLPs from which four are expressed during WBD progression. One of them is similar to small TLPs, which are present in phytopathogenic basidiomycete, such as wheat stem rust fungus Puccinia graminis. Fungi genomes annotation and phylogenetic data revealed a larger number of TLPs in basidiomycetes when comparing with ascomycetes, suggesting that these proteins could be involved in specific traits of mushroom-forming species. Based on the present data, we discuss the contribution of TLPs in the combat against fungal competitors and hypothesize a role of these proteins in M. perniciosa pathogenicity. (C) 2015 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 10/52636-8 - Caracterizacao funcional e estrutural de proteinas similares a proteinas pr-1 expressas por moniliophthora perniciosa, fungo causador da vassoura de bruxa do cacaueiro.
• Beneficiário: Renata Moro Baroni
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/50810-3 - Caracterizacao de proteinas similares a taumatinas em fungos causadores de doencas em cacau, monilophthora
• Beneficiário: Sulamita de Freitas Franco
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/50119-9 - Estudo integrado e comparativo de três doenças fúngicas do cacau: vassoura-de-bruxa, monilíase e mal do facão, visando à compreensão de mecanismos de patogenicidade para o desenvolvimento de estratégias de controle
• Beneficiário: Gonçalo Amarante Guimarães Pereira
• Modalidade de apoio: Auxílio à Pesquisa - Temático