Monoamine oxidase B layer-by-layer film fabrication and characterization toward dopamine detection

Autor(es)

Miyazaki, Celina Massumi ^[1] ; Pereira, Tamyris Paschoal ^[1] ; Tavares Mascagni, Daniela Branco ^[2] ; de Moraes, Marli Leite ^[3] ; Ferreira, Marystela ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, UFSCar, CCTS, Sao Paulo - Brazil

^[2] Univ Estadual Sao Paulo UNESP, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, UNIFESP, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Materials Science & Engineering C-Materials for Biological Applications; v. 58, p. 310-315, JAN 1 2016.
• Citações Web of Science: 12

Resumo

In this work nanostructured film composites of the monoamine oxidase B (MAO-B) enzyme, free or encapsulated in liposomes, were fabricated by the layer-by-layer (LbL) self-assembly technique, employing polyethylene imine (PEI) as polycation. Initially, the MAO-B enzyme was incorporated into liposomes in order to preserve its enzymatic structure ensuring their activity and catalytic stability. The LbL film growth was monitored by surface plasmon resonance (SPR) by gold resonance angle shift analysis after each bilayer deposition. Subsequently, the films were applied as amperometric biosensors for dopamine detection using Prussian Blue (PB) as the electron mediator. The biosensor fabricated by MAO-B incorporated into liposomes composed of DPPG:POPG in the ratio (1:4) (w/w) showed the best performance with a sensitivity of 0.86 (mu A cm(-2))/(mmol L-1) and a detection limit of 033 mmol L-1. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 14/15093-7 - Estudos de sistemas nanoestruturados por ressonância plasmônica de superfície (SPR): aplicações em biossensoriamento
• Beneficiário: Celina Massumi Miyazaki
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/17519-1 - Imobilização de nanopartículas metálicas em filmes ultrafinos visando ao biossensoriamento
• Beneficiário: Marystela Ferreira
• Modalidade de apoio: Auxílio à Pesquisa - Regular