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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Monoamine oxidase B layer-by-layer film fabrication and characterization toward dopamine detection

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Autor(es):
Miyazaki, Celina Massumi [1] ; Pereira, Tamyris Paschoal [1] ; Tavares Mascagni, Daniela Branco [2] ; de Moraes, Marli Leite [3] ; Ferreira, Marystela [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Fed Sao Carlos, UFSCar, CCTS, Sao Paulo - Brazil
[2] Univ Estadual Sao Paulo UNESP, Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, UNIFESP, Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Materials Science & Engineering C-Materials for Biological Applications; v. 58, p. 310-315, JAN 1 2016.
Citações Web of Science: 12
Resumo

In this work nanostructured film composites of the monoamine oxidase B (MAO-B) enzyme, free or encapsulated in liposomes, were fabricated by the layer-by-layer (LbL) self-assembly technique, employing polyethylene imine (PEI) as polycation. Initially, the MAO-B enzyme was incorporated into liposomes in order to preserve its enzymatic structure ensuring their activity and catalytic stability. The LbL film growth was monitored by surface plasmon resonance (SPR) by gold resonance angle shift analysis after each bilayer deposition. Subsequently, the films were applied as amperometric biosensors for dopamine detection using Prussian Blue (PB) as the electron mediator. The biosensor fabricated by MAO-B incorporated into liposomes composed of DPPG:POPG in the ratio (1:4) (w/w) showed the best performance with a sensitivity of 0.86 (mu A cm(-2))/(mmol L-1) and a detection limit of 033 mmol L-1. (C) 2015 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 14/15093-7 - Estudos de sistemas nanoestruturados por ressonância plasmônica de superfície (SPR): aplicações em biossensoriamento
Beneficiário:Celina Massumi Miyazaki
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 14/17519-1 - Imobilização de nanopartículas metálicas em filmes ultrafinos visando ao biossensoriamento
Beneficiário:Marystela Ferreira
Linha de fomento: Auxílio à Pesquisa - Regular