Monoamine oxidase B layer-by-layer film fabrication and characterization toward dopamine detection

Author(s)

Miyazaki, Celina Massumi ^[1] ; Pereira, Tamyris Paschoal ^[1] ; Tavares Mascagni, Daniela Branco ^[2] ; de Moraes, Marli Leite ^[3] ; Ferreira, Marystela ^[1]

Affiliation

^[1] Univ Fed Sao Carlos, UFSCar, CCTS, Sao Paulo - Brazil

^[2] Univ Estadual Sao Paulo UNESP, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, UNIFESP, Sao Paulo - Brazil

• Document type: Journal article
• Source: Materials Science & Engineering C-Materials for Biological Applications; v. 58, p. 310-315, JAN 1 2016.
• Web of Science Citations: 12

Abstract

In this work nanostructured film composites of the monoamine oxidase B (MAO-B) enzyme, free or encapsulated in liposomes, were fabricated by the layer-by-layer (LbL) self-assembly technique, employing polyethylene imine (PEI) as polycation. Initially, the MAO-B enzyme was incorporated into liposomes in order to preserve its enzymatic structure ensuring their activity and catalytic stability. The LbL film growth was monitored by surface plasmon resonance (SPR) by gold resonance angle shift analysis after each bilayer deposition. Subsequently, the films were applied as amperometric biosensors for dopamine detection using Prussian Blue (PB) as the electron mediator. The biosensor fabricated by MAO-B incorporated into liposomes composed of DPPG:POPG in the ratio (1:4) (w/w) showed the best performance with a sensitivity of 0.86 (mu A cm(-2))/(mmol L-1) and a detection limit of 033 mmol L-1. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 14/15093-7 - Study of nanostructured systems by surface plasmon resonance (SPR): applications in biosensing
• Grantee: Celina Massumi Miyazaki
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 14/17519-1 - Immobilization of metal nanoparticules in ultrathin films aiming biosensing
• Grantee: Marystela Ferreira
• Support Opportunities: Regular Research Grants