Antimicrobial activity of Chlorhexidine, Peracetic acid and Sodium hypochlorite/etidronate irrigant solutions against Enterococcus faecalis biofilms

Autor(es)

Arias-Moliz, M. T. ^[1] ; Ordinola-Zapata, R. ^[2] ; Baca, P. ^[3] ; Ruiz-Linares, M. ^[3] ; Garcia, E. Garcia ^[3] ; Duarte, M. A. Hungaro ^[2] ; Bramante, C. Monteiro ^[2] ; Ferrer-Luque, C. M. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Granada, Sch Dent, Dept Microbiol, Granada - Spain

^[2] Univ Sao Paulo, Bauru Dent Sch, Dept Endodont, Bauru - Brazil

^[3] Univ Granada, Sch Dent, Dept Stomatol, Granada - Spain

• Tipo de documento: Artigo Científico
• Fonte: International Endodontic Journal; v. 48, n. 12, p. 1188-1193, DEC 2015.
• Citações Web of Science: 21

Resumo

AimTo evaluate the antimicrobial effect of 2.5% sodium hypochlorite alone (NaOCl) and associated with 9% HEBP (NaOCl/HEBP), 2% peracetic acid (PAA) and 2% chlorhexidine (CHX), on the viability of Enterococcus faecalis biofilms attached to dentine. MethodologyBiofilms of E.faecalis were grown on the surface of dentine blocks for 5days and then exposed to the irrigating solutions for 3min. Distilled water was used as the control. The total biovolume and the percentage of dead cells of the infected dentine were measured by means of confocal microscopy and the live/dead technique. Nonparametric tests were used to determine statistical differences (P<0.05). ResultsNaOCl and the NaOCl/HEBP mixture were associated with a significantly greater percentage of dead cells, followed by PAA (P<0.05). No significant antimicrobial effect of CHX was observed in comparison with the control group. Total biovolume decreased significantly in NaOCl, NaOCl/HEBP and PAA solutions in comparison with the CHX and control groups. ConclusionsNaOCl alone or associated with HEBP were the most effective irrigant solutions in dissolving and killing E.faecalis biofilms. (AU)

• Processo FAPESP: 13/03695-0 - Avaliação da anatomia, protocolos de instrumentação e de descontaminação de raízes por meio de microtomografia, prototipagem de alta resolução, microscopia óptica e confocal.
• Beneficiário: Ronald Ordinola Zapata
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado