Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Antimicrobial activity of Chlorhexidine, Peracetic acid and Sodium hypochlorite/etidronate irrigant solutions against Enterococcus faecalis biofilms

Full text
Author(s):
Arias-Moliz, M. T. [1] ; Ordinola-Zapata, R. [2] ; Baca, P. [3] ; Ruiz-Linares, M. [3] ; Garcia, E. Garcia [3] ; Duarte, M. A. Hungaro [2] ; Bramante, C. Monteiro [2] ; Ferrer-Luque, C. M. [3]
Total Authors: 8
Affiliation:
[1] Univ Granada, Sch Dent, Dept Microbiol, Granada - Spain
[2] Univ Sao Paulo, Bauru Dent Sch, Dept Endodont, Bauru - Brazil
[3] Univ Granada, Sch Dent, Dept Stomatol, Granada - Spain
Total Affiliations: 3
Document type: Journal article
Source: International Endodontic Journal; v. 48, n. 12, p. 1188-1193, DEC 2015.
Web of Science Citations: 21
Abstract

AimTo evaluate the antimicrobial effect of 2.5% sodium hypochlorite alone (NaOCl) and associated with 9% HEBP (NaOCl/HEBP), 2% peracetic acid (PAA) and 2% chlorhexidine (CHX), on the viability of Enterococcus faecalis biofilms attached to dentine. MethodologyBiofilms of E.faecalis were grown on the surface of dentine blocks for 5days and then exposed to the irrigating solutions for 3min. Distilled water was used as the control. The total biovolume and the percentage of dead cells of the infected dentine were measured by means of confocal microscopy and the live/dead technique. Nonparametric tests were used to determine statistical differences (P<0.05). ResultsNaOCl and the NaOCl/HEBP mixture were associated with a significantly greater percentage of dead cells, followed by PAA (P<0.05). No significant antimicrobial effect of CHX was observed in comparison with the control group. Total biovolume decreased significantly in NaOCl, NaOCl/HEBP and PAA solutions in comparison with the CHX and control groups. ConclusionsNaOCl alone or associated with HEBP were the most effective irrigant solutions in dissolving and killing E.faecalis biofilms. (AU)

FAPESP's process: 13/03695-0 - Analysis of the anatomy, shaping and disinfection procedures on roots by microcomputed tomography, rapid prototyping, optical and confocal microscopy study.
Grantee:Ronald Ordinola Zapata
Support Opportunities: Scholarships in Brazil - Post-Doctoral