Bioactive Cyanopeptides Produced by Sphaerocavum brasiliense Strains (Cyanobacteria)

Autor(es)

Silva-Stenico, Maria Estela ^[1] ; Rigonato, Janaina ^[1] ; Lorenzi, Adriana Sturion ^[1] ; Paiva Azevedo, Maria Teresa ^[2] ; Sant'Anna, Celia Leite ^[3] ; Fiore, Marli Fatima ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, CENA, Lab Ecol Cianobacterias, BR-13400970 Piracicaba, SP - Brazil

^[2] Bioalgas, BR-04304000 Sao Paulo, SP - Brazil

^[3] Inst Bot Estado Sao Paulo, BR-04301012 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 26, n. 10, p. 2088-2096, OCT 2015.
• Citações Web of Science: 2

Resumo

The halogenated compounds aeruginosin and cyanopeptolin (protease inhibitors) are well documented in several cyanobacteria. In this work, the presence of genetic coding for aeruginosin and cyanopeptolin in Sphaerocavum genus and their characterization by mass spectrometry and potential toxic effects were investigated. Three strains of Sphaerocavum brasiliense (CCIBt 3094, CCIBt 3096 and CCIBt 3316) isolated from different reservoirs in Sao Paulo State, and one (CCIBt 3179) from Rio Grande do Norte State were selected for this study. Fragments of aerA-aerB and mcnC-mcnE gene regions (aeruginosin and cyanopeptolin, respectively) were amplified by polymerase chain reaction (PCR) and sequenced. Compounds were characterized by mass spectrometry. All the studied strains presented aeruginosin and cyanopeptolin synthetase genes. Mass spectrometry showed the presence of several aeruginosin and cyanopeptolin variants. The Allium test showed that the ethanolic extract promoted toxic and cytotoxic actions in acute and chronic treatments. These naturally occurring protease inhibitors can play an important role in the physiopathology of human diseases. (AU)

• Processo FAPESP: 09/05474-5 - Bioprospecção de cianobactéria: seleção de cianopeptídeos para aplicação farmacêutica e biotecnológica
• Beneficiário: Maria Estela Stenico
• Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
• Processo FAPESP: 10/09867-9 - Bioprospecção de cianobactéria: seleção de cianopeptídeos para aplicação farmacêutica e biotecnológica
• Beneficiário: Maria Estela Stenico
• Linha de fomento: Bolsas no Brasil - Apoio a Jovens Pesquisadores
• Processo FAPESP: 08/53627-2 - Aplicação da técnica de qPCR para o monitoramento de cianobactérias e suas toxinas em reservatórios de água
• Beneficiário: Adriana Sturion Lorenzi
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado