Quantitative proteomic analysis of Araucaria angustzfolia (Bertol.) Kuntze cell lines with contrasting embryogenic potential

Autor(es)

Wendt dos Santos, Andre Luis ^[1] ; Elbl, Paula ^[1] ; Navarro, Bruno Viana ^[1] ; de Oliveira, Leandro Francisco ^[1] ; Salvato, Fernando ^[2] ; Balbuena, Tiago Santana ^[3] ; Segal Floh, Eny Iochevet ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biosci, Dept Bot, BR-05508090 Sao Paulo, SP - Brazil

^[2] Univ Estadual Campinas, Inst Biol, Dept Plant Biol, Campinas, SP - Brazil

^[3] Sao Paulo State Univ, Coll Agr & Vet Sci, Jaboticabal, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PROTEOMICS; v. 130, p. 180-189, JAN 1 2016.
• Citações Web of Science: 13

Resumo

GeLC-MS/MS based label free proteomic profiling was used in the large scale identification and quantification of proteins from Brazilian pine (Araucaria angustifolia) embryogenic cell (EC) lines that showed different propensities to form somatic embryos. Using a predicted protein sequence database that was derived from A. angusafolia RNA-Seq data, 2398 non-redundant proteins were identified. The log(2) of the spectral count values of 858 proteins of these proteins showed a normal distribution, and were used for statistical analysis. Statistical tests indicated that 106 proteins were significantly differentially abundant between the two EC lines, and that 35 were more abundant in the responsive genotype (EC line SE1) and 71 were more abundant in the blocked genotype (EC line SE6). An increase in the abundance of proteins related to cell defense, anti-oxidative stress responses, and storage reserve deposition was observed in SEL Moreover, in SE6 we observed an increased abundance of two proteins associated with seed development during the embryogenic cell proliferation stage, which we suggest is associated with genotypes showing a low responsiveness to embryo formation. Differences in protein abundance between the EC lines are discussed in terms of carbohydrate metabolism, cell division, defense response, gene expression, and response to reactive oxygen species. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 14/26888-0 - Marcadores bioquímicos associados ao metabolismo de carboidratos durante a embriogênese zigótica e somática de Araucaria angustifolia (Bert.) O. Ktze.
• Beneficiário: Bruno Viana Navarro
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 12/51145-6 - Aminoácidos, poliaminas e óxido nítrico como moduladores da poliembriogênese zigótica e somática em Araucaria angustifolia (Bert) O. Ktze.
• Beneficiário: André Luiz Wendt dos Santos
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado