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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Quantitative proteomic analysis of Araucaria angustzfolia (Bertol.) Kuntze cell lines with contrasting embryogenic potential

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Author(s):
Wendt dos Santos, Andre Luis [1] ; Elbl, Paula [1] ; Navarro, Bruno Viana [1] ; de Oliveira, Leandro Francisco [1] ; Salvato, Fernando [2] ; Balbuena, Tiago Santana [3] ; Segal Floh, Eny Iochevet [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Inst Biosci, Dept Bot, BR-05508090 Sao Paulo, SP - Brazil
[2] Univ Estadual Campinas, Inst Biol, Dept Plant Biol, Campinas, SP - Brazil
[3] Sao Paulo State Univ, Coll Agr & Vet Sci, Jaboticabal, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF PROTEOMICS; v. 130, p. 180-189, JAN 1 2016.
Web of Science Citations: 13
Abstract

GeLC-MS/MS based label free proteomic profiling was used in the large scale identification and quantification of proteins from Brazilian pine (Araucaria angustifolia) embryogenic cell (EC) lines that showed different propensities to form somatic embryos. Using a predicted protein sequence database that was derived from A. angusafolia RNA-Seq data, 2398 non-redundant proteins were identified. The log(2) of the spectral count values of 858 proteins of these proteins showed a normal distribution, and were used for statistical analysis. Statistical tests indicated that 106 proteins were significantly differentially abundant between the two EC lines, and that 35 were more abundant in the responsive genotype (EC line SE1) and 71 were more abundant in the blocked genotype (EC line SE6). An increase in the abundance of proteins related to cell defense, anti-oxidative stress responses, and storage reserve deposition was observed in SEL Moreover, in SE6 we observed an increased abundance of two proteins associated with seed development during the embryogenic cell proliferation stage, which we suggest is associated with genotypes showing a low responsiveness to embryo formation. Differences in protein abundance between the EC lines are discussed in terms of carbohydrate metabolism, cell division, defense response, gene expression, and response to reactive oxygen species. (C) 2015 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 14/26888-0 - Biochemical markers associated with carbohydrate metabolism during Araucaria angustifolia zygotic and somatic embryogenesis
Grantee:Bruno Viana Navarro
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 12/51145-6 - Amino acids, polyamines and nitric oxide as modulators of zygotic and somatic polyembryogenesis in Araucaria angustifolia (Bert) O. Ktze.
Grantee:André Luiz Wendt dos Santos
Support Opportunities: Scholarships in Brazil - Post-Doctoral