Expression Profiles of Cytokine mRNAs in the Pleural Fluid Reveal Differences Among Tuberculosis, Malignancies, and Pneumonia-Exudative Pleural Effusions

Autor(es)

Alves Esposito, Danillo Lucas ^[1] ; Bollela, Valdes Roberto ^[1] ; Pereira Feitosa, Ana Luisa ^[1] ; Lopes da Fonseca, Benedito Antonio ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Internal Med, Sch Med Ribeirao Preto, Div Infect Dis, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Dept Internal Med, Sch Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lung; v. 193, n. 6, p. 1001-1007, DEC 2015.
• Citações Web of Science: 2

Resumo

Introduction Tuberculosis (TB) and malignant diseases are the most common causes of lymphocytic pleural effusion in adults. Serum and pleural fluid cytokine levels have been analyzed to help in the differential diagnosis, but with limited results. Purpose This study investigates transcription levels of selected cytokine genes in pleural effusion of patients under investigation for TB. Methods This was a prospective study that included adult patients under investigation for pleural effusion in Brazil. The expression of 19 cytokine genes was analyzed by RT-qPCR. Results The majority of cytokine-related genes expressed in pleural fluid of TB patients were similar in non-TB patients, except for RORA and RORC genes, which showed a statistically higher level in TB. All cytokines in the Th17 pattern were induced in TB patients' pleural fluid. Patients with malignant pleural effusion expressed higher levels of IFN-alpha 1, IFN-beta 1, TNF-alpha, IL-4 and IL-6, and suppression of TGF beta-1. Conclusion There is still a lot to understand about the cytokine roles in the pro- and anti-inflammatory environment of exudative pleural effusions. The data presented here showed an increased expression of Th17 pattern cytokines genes in TB patients that could be used as markers to differentiate tuberculous pleuritis from other common causes of exudative pleural effusion. (AU)

• Processo FAPESP: 09/17305-3 - Análise quantitativa da expressão de RNAs mensageiros de citocinas inflamatórias em células do líquido pleural de pacientes com suspeita de tuberculose pleural
• Beneficiário: Benedito Antônio Lopes da Fonseca
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/03505-0 - Análise quantitativa da expressão de RNAs mensageiros de citocinas inflamatórias em células do líquido pleural de pacientes com suspeita de tuberculose pleural.
• Beneficiário: Ana Luisa Pereira Feitosa
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico