Nanofilms of hyaluronan/chitosan assembled layer-by-layer: An antibacterial surface for Xylella fastidiosa

Autor(es)

Hernandez-Montelongo, Jacobo ^[1] ; Nascimento, Vicente F. ^[2] ; Murillo, Duber ^[1] ; Taketa, Thiago B. ^[2] ; Sahoo, Prasana ^[1] ; de Souza, Alessandra A. ^[3] ; Beppu, Marisa M. ^[2] ; Cotta, Monica A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Fis Gleb Wataghin, Dept Fis Aplicada, BR-13083859 Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Fac Engn Quim, BR-13083852 Sao Paulo - Brazil

^[3] Ctr Apta Citros Sylvio Moreira, Inst Agron Campinas, BR-13490970 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Carbohydrate Polymers; v. 136, p. 1-11, JAN 20 2016.
• Citações Web of Science: 19

Resumo

In this work, nanofilms of hyaluronan/chitosan (HA/CHI) assembled layer by layer were synthesized; their application as a potential antimicrobial material was demonstrated for the phytopathogen Xylella fastidiosa, a gram-negative bacterium, here used as a model. For the synthesis, the influence of pH and ionic strength of these natural polymer stem-solutions on final characteristics of the HA/CHI nanofilms was studied in detail. The antibacterial effect was evaluated using widefield fluorescence microscopy. These results were correlated with the chemical properties of the nanofilms, studied by FTIR and Raman spectroscopy, as well as with their morphology and surface properties characterized using SEM and AFM. The present findings can be extended to design and optimize HA/CHI nanofilms with enhanced antimicrobial behavior for other type of phytopathogenic gram-negative bacteria species, such as Xanthomonas citri, Xanthomas campestri and Ralstonia solanacearum. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 13/02300-1 - Nanofios semicondutores: mecanismos de formação e aplicação em biossensores
• Beneficiário: Mônica Alonso Cotta
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/51748-7 - Análise estrutural e química de biofilmes de Xylella fastidiosa
• Beneficiário: Mônica Alonso Cotta
• Modalidade de apoio: Auxílio à Pesquisa - Regular