| Full text | |
| Author(s): |
Hernandez-Montelongo, Jacobo
[1]
;
Nascimento, Vicente F.
[2]
;
Murillo, Duber
[1]
;
Taketa, Thiago B.
[2]
;
Sahoo, Prasana
[1]
;
de Souza, Alessandra A.
[3]
;
Beppu, Marisa M.
[2]
;
Cotta, Monica A.
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Estadual Campinas, Inst Fis Gleb Wataghin, Dept Fis Aplicada, BR-13083859 Sao Paulo - Brazil
[2] Univ Estadual Campinas, Fac Engn Quim, BR-13083852 Sao Paulo - Brazil
[3] Ctr Apta Citros Sylvio Moreira, Inst Agron Campinas, BR-13490970 Sao Paulo - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Carbohydrate Polymers; v. 136, p. 1-11, JAN 20 2016. |
| Web of Science Citations: | 19 |
| Abstract | |
In this work, nanofilms of hyaluronan/chitosan (HA/CHI) assembled layer by layer were synthesized; their application as a potential antimicrobial material was demonstrated for the phytopathogen Xylella fastidiosa, a gram-negative bacterium, here used as a model. For the synthesis, the influence of pH and ionic strength of these natural polymer stem-solutions on final characteristics of the HA/CHI nanofilms was studied in detail. The antibacterial effect was evaluated using widefield fluorescence microscopy. These results were correlated with the chemical properties of the nanofilms, studied by FTIR and Raman spectroscopy, as well as with their morphology and surface properties characterized using SEM and AFM. The present findings can be extended to design and optimize HA/CHI nanofilms with enhanced antimicrobial behavior for other type of phytopathogenic gram-negative bacteria species, such as Xanthomonas citri, Xanthomas campestri and Ralstonia solanacearum. (C) 2015 Elsevier Ltd. All rights reserved. (AU) | |
| FAPESP's process: | 13/02300-1 - Semiconductor nanowires: formation mechanisms and biosensing applications |
| Grantee: | Mônica Alonso Cotta |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 10/51748-7 - Chemical and structural analysis of Xylella fastidiosa biofilms |
| Grantee: | Mônica Alonso Cotta |
| Support Opportunities: | Regular Research Grants |