Lack of association among TNF-alpha gene expression,-308 polymorphism (G > A) and virulence markers of Helicobacter pylori

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Zabaglia, Luanna Munhoz ^[1] ; Ferraz, Mariane Avante ^[1] ; Pereira, Weendelly Nayara ^[1] ; Orcini, Wilson Aparecido ^[1] ; de Labio, Roger Willian ^[2] ; Neto, Agostinho Caleman ^[2] ; Wisnieski, Fernanda ^[3] ; de Oliveira, Juliana Garcia ^[1] ; Cardoso Smith, Marilia de Arruda ^[3] ; Marques Payao, Spencer Luiz ^{[1, 2]} ; Rasmussen, Lucas Trevizani ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sagrado Coracao, BR-17011160 Bauru, SP - Brazil

^[2] Fac Med Marilia, BR-17519050 Marilia, SP - Brazil

^[3] Univ Fed Sao Paulo, BR-04021001 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Venomous Animals and Toxins including Tropical Diseases; v. 21, DEC 30 2015.
• Citações Web of Science: 4

Resumo

Background: Tumor necrosis factor plays a critical role in the pathogenesis of gastric diseases such as gastric cancer, and an abnormal inflammatory response has frequently been observed in dyspeptic patients. Helicobacter pylori infection can induce a gastric mucosal inflammatory response that may be influenced by -308 (G > A) polymorphisms and gene expression of the TNF-alpha gene. Methods: One hundred and thirty-four gastric biopsy samples were collected from patients of both genders (61 male and 73 female, mean age 40.3 +/- 24.2 years) with gastric symptoms. The -308 (G > A) polymorphism of TNF-alpha was characterized using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). The expression level was measured using real-time PCR, and relative quantification (RQ) was calculated using the comparative CT method (2(Delta Delta CT)). Results: The analysis revealed an increase in TNF-alpha gene expression in patients with gastritis; on the other hand, no statistical differences were observed in patients with gastric cancer. In addition, no association was found among -308 polymorphism genotypes, virulence markers, or TNF-alpha gene expression. Conclusions: Helicobacter pylori induces a large increase in TNF-alpha expression in patients with gastritis, regardless of tissue inflammation, but after the tissue becomes neoplastic, the presence of bacteria did not influence expression. These results suggest that the TNF-alpha pathway may play an important role in the progression from gastritis to gastric cancer (AU)

• Processo FAPESP: 12/18333-3 - Caracterização do marcador de patogenicidade dupA, do Helicobacter pylori, genotipagem e análise da expressão dos genes do fator de necrose tumoral e E-caderina em amostras de crianças e adultos com sintomas pépticos
• Beneficiário: Lucas Trevizani Rasmussen
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/21224-4 - Genotipagem e análise da expressão do gene TNF-alpha em amostras de pacientes com sintomas pépticos e Câncer gástrico: uma possível relação com o Helicobacter pylori
• Beneficiário: Luanna Munhoz Zabaglia
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 13/21285-3 - Caracterização do marcador de patogenicidade dupA, do Helicobacter pylori e análise da expressão do gene da E-caderina em amostras de biopsia gástrica de pacientes dispépticos e com adenocarcinoma gástrico
• Beneficiário: Mariane Avante Ferraz
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica