Simultaneous encapsulation of magnetic nanoparticles and zinc phthalocyanine in poly(methyl methacrylate) nanoparticles by miniemulsion polymerization and in vitro studies

Autor(es)

Feuser, Paulo Emilio ^[1] ; Fernandes, Andrielle Castilho ^[2] ; Nele, Marcio ^[3] ; Viegas, Alexandre da Cas ^[4] ; Ricci-Junior, Eduardo ^[5] ; Tedesco, Antonio Claudio ^[2] ; Sayer, Claudia ^[1] ; Hermes de Araujo, Pedro Henrique ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Santa Catarina, Dept Chem Engn & Food Engn, BR-88010970 Florianopolis, SC - Brazil

^[2] Univ Sao Paulo, Ctr Nanotechnol & Tissue Engineers Photobiol & Ph, Sao Paulo - Brazil

^[3] Univ Fed Rio de Janeiro, Sch Chem, BR-21941 Rio De Janeiro - Brazil

^[4] Univ Fed Santa Catarina, Dept Phys, BR-88040900 Florianopolis, SC - Brazil

^[5] Univ Fed Rio de Janeiro, Fac Pharm, BR-21941 Rio De Janeiro - Brazil

• Tipo de documento: Artigo Científico
• Fonte: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 135, p. 357-364, NOV 1 2015.
• Citações Web of Science: 14

Resumo

The aim of this work was the simultaneous encapsulation of magnetic nanoparticles (MNPs) and zinc(II) phthalocyanine (ZnPc) in poly(methyl methacrylate) (PMMA) (MNPsZnPc-PMMA) nanoparticles (NPs) by miniemulsion polymerization and to evaluate the photobiological activity and/or hyperthermia (HPT) against human glioblastoma cells (U87MG). MNPsZnPc-PMMA NPs presented an average diameter of 104 +/- 2.5 nm with a polydispersity index (PdI) of 0.14 +/- 0.03 and negative surface charge -47 +/- 2.2 mV (pH 7.4 +/- 0.1). The encapsulation efficiency (EE%) of ZnPc was 85.7% +/- 1.30. The release of ZnPc from PMMA NPs was slow and sustained without the presence of burst effect, indicating a homogeneous distribution of the drug in the polymeric matrix. In the biological assay, MNPsZnPc-PMMA NPs showed considerable cytotoxic effect on U87MG cells only after activation with visible light at 675 nm (photodynamic therapy, PDT) or after application of an alternating magnetic field. The simultaneous encapsulation of MNPs and ZnPc in a drug delivery system with sustained release can be a new alternative for cancer treatment leading to significant tumor regression after minimum doses of heat dissipation and light. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 08/53719-4 - Utilização de nanocarreadores contendo fármacos fotossensíveis e outros ativos derivados de fatores de crescimento aplicados a processos fotodinâmicos nas áreas da saúde
• Beneficiário: Antonio Claudio Tedesco
• Modalidade de apoio: Auxílio à Pesquisa - Temático