LEEK YELLOW STRIPE VIRUS ISOLATES FROM BRAZIL FORM A DISTINCT CLADE BASED ON THE P1 GENE

Autor(es)

Bampi, D. ^{[1, 2]} ; Mituti, T. ^[3] ; Pavan, M. A. ^[1] ; Hammond, J. ^[2] ; Krause-Sakate, R. ^[1]

Afiliação do(s) autor(es)

^[1] UNESP, FCA, Dept Protecao Vegetal, BR-18610307 Botucatu, SP - Brazil

^[2] ARS, USDA, USNA, Floral & Nursery Plants Res Unit, Beltsville, MD 20705 - USA

^[3] Univ Sao Paulo, ESALQ, Dept Fitopatol & Nematol, BR-13418900 Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PLANT PATHOLOGY; v. 97, n. 3, p. 457-463, NOV 2015.
• Citações Web of Science: 1

Resumo

The complete genome sequence of a garlic isolate of Leek yellow stripe virus (LYSV) from Brazil (LYSV-MG) was determined and its phylogenetic relationships with other LYSV isolates from other parts of the world were inferred. The LYSV-MG genome consists of 10,341 nucleotides and encodes a polyprotein of 3,221 amino acids. Brazilian LYSV isolates are more closely related to S-type than to N-type isolates but do not have a deletion in the P1 gene. Partial nucleotide sequence analysis of the P1 gene revealed 97-99% identity among Brazilian isolates, and 51-64% identity between Brazilian isolates and isolates from other countries for which sequence information is available in GenBank. The Brazilian isolates formed a monophyletic group closest to the S-type isolates and one isolate from Okinawa (O-type). These data suggest that Brazilian LYSV isolates are derived from an ancestral source of the Okinawa and S-type isolates, prior to the occurrence of the P1 deletion and divergence of the S-type isolates. (AU)

• Processo FAPESP: 10/16148-9 - Levantamento e caracterização dos principais Potyvirus e Carlavirus ocorrentes em alho, sequenciamento do genoma completo do Leek yellow stripe vírus (LYSV) e desenvolvimento de técnicas para detecção de Shallot latent vírus (SLV)
• Beneficiário: Marcelo Agenor Pavan
• Modalidade de apoio: Auxílio à Pesquisa - Regular