Liposome-Loaded Cell Backpacks

Autor(es)

Polak, Roberta ^{[1, 2]} ; Lim, Rosanna M. ^[3] ; Beppu, Marisa M. ^[4] ; Pitombo, Ronaldo N. M. ^[2] ; Cohen, Robert E. ^[3] ; Rubner, Michael F. ^[1]

Afiliação do(s) autor(es)

^[1] MIT, Dept Mat Sci & Engn, Cambridge, MA 02139 - USA

^[2] Univ Sao Paulo, Sch Pharmaceut Sci, BR-05508900 Sao Paulo, SP - Brazil

^[3] MIT, Dept Chem Engn, Cambridge, MA 02139 - USA

^[4] Univ Estadual Campinas, UNICAMP, Sch Chem Engn, BR-13083852 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ADVANCED HEALTHCARE MATERIALS; v. 4, n. 18, p. 2832-2841, DEC 30 2015.
• Citações Web of Science: 14

Resumo

Cell backpacks, or micron-scale patches of a few hundred nanometers in thickness fabricated by layer-by-layer (LbL) assembly, are potentially useful vehicles for targeted drug delivery on the cellular level. In this work, echogenic liposomes (ELIPs) containing the anticancer drug doxorubicin (DOX) are embedded into backpacks through electrostatic interactions and LbL assembly. Poly(allylamine hydrochloride)/poly(acrylic acid) (PAH/PAA)(n), and poly(diallyldimethylammonium chloride)/poly(styrene sulfonate) (PDAC/SPS)(n) film systems show the greatest ELIP incorporation of the films studied while maintaining the structural integrity of the vesicles. The use of ELIPs for drug encapsulation into backpacks facilitates up to three times greater DOX loading compared to backpacks without ELIPs. Cytotoxicity studies reveal that monocyte backpack conjugates remain viable even after 72 h, demonstrating promise as drug delivery vehicles. Because artificial vesicles can load many different types of drugs, ELIP containing backpacks offer a unique versatility for broadening the range of possible applications for cell backpacks. (AU)

• Processo FAPESP: 10/51066-3 - Desenvolvimento e fabricacao de filmes ultra-finos, obtidos pela tecnica layer-by-layer, para aplicacoes na entrega direcionada de farmacos e na captura seletiva de bio-marcadores.
• Beneficiário: Roberta Polak
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/21326-6 - Desenvolvimento de filme funcional via técnica de layer-by-layer para liberação de droga antineoplásica encapsulada em lipossomas ecogênicos
• Beneficiário: Roberta Polak
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado