Liposome-Loaded Cell Backpacks

Author(s)

Polak, Roberta ^{[1, 2]} ; Lim, Rosanna M. ^[3] ; Beppu, Marisa M. ^[4] ; Pitombo, Ronaldo N. M. ^[2] ; Cohen, Robert E. ^[3] ; Rubner, Michael F. ^[1]

Affiliation

^[1] MIT, Dept Mat Sci & Engn, Cambridge, MA 02139 - USA

^[2] Univ Sao Paulo, Sch Pharmaceut Sci, BR-05508900 Sao Paulo, SP - Brazil

^[3] MIT, Dept Chem Engn, Cambridge, MA 02139 - USA

^[4] Univ Estadual Campinas, UNICAMP, Sch Chem Engn, BR-13083852 Campinas, SP - Brazil

• Document type: Journal article
• Source: ADVANCED HEALTHCARE MATERIALS; v. 4, n. 18, p. 2832-2841, DEC 30 2015.
• Web of Science Citations: 14

Abstract

Cell backpacks, or micron-scale patches of a few hundred nanometers in thickness fabricated by layer-by-layer (LbL) assembly, are potentially useful vehicles for targeted drug delivery on the cellular level. In this work, echogenic liposomes (ELIPs) containing the anticancer drug doxorubicin (DOX) are embedded into backpacks through electrostatic interactions and LbL assembly. Poly(allylamine hydrochloride)/poly(acrylic acid) (PAH/PAA)(n), and poly(diallyldimethylammonium chloride)/poly(styrene sulfonate) (PDAC/SPS)(n) film systems show the greatest ELIP incorporation of the films studied while maintaining the structural integrity of the vesicles. The use of ELIPs for drug encapsulation into backpacks facilitates up to three times greater DOX loading compared to backpacks without ELIPs. Cytotoxicity studies reveal that monocyte backpack conjugates remain viable even after 72 h, demonstrating promise as drug delivery vehicles. Because artificial vesicles can load many different types of drugs, ELIP containing backpacks offer a unique versatility for broadening the range of possible applications for cell backpacks. (AU)

• FAPESP's process: 11/21326-6 - Development of functional patches via layer-by-layer for the release of anticancer drug encapsulated in echogenic liposomes
• Grantee: Roberta Polak
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate