Development of functional patches via layer-by-layer for the release of anticancer drug encapsulated in echogenic liposomes

• Grant number: 11/21326-6
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: May 01, 2012
• End date: April 30, 2013
• Field of knowledge: Engineering - Biomedical Engineering - Medical Engineering
• Principal Investigator: Ronaldo Nogueira de Moraes Pitombo
• Grantee: Roberta Polak
• Supervisor: Michael F. Rubner
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: Massachusetts Institute of Technology (MIT), United States

Abstract

In this project the incorporation of an antitumor drug (vincristine) trapped in echogenic liposomes (ELIP)and embedded into polyelectrolyte multilayer films (PEM), will be studied. These films will be obtained by layer-by-layer technique. The aim is to check the possibilities of incorporating this drug to the films (trapped in ELIP or not) and to characterize the films obtained through the techniques of scanning electron microscopy (SEM), atomic force microscopy (AFM), profilometry (to check thickness and roughness of the films), incorporation of dyes (to check the homogeneity of the films and location of ionic terminals) and testing of drug release in PBS (phosphate buffered saline). These films have potential application for functionalization of immune system cells (eg T cells and macrophages). For this purpose, the period of study abroad will also be held in the training culture of these cell types for subsequent functionalization of the same. Therefore, the PhD sandwich will be extremely important to complement studies that have been conducted so far in Brazil (FCF / USP-SP and FEQ / UNICAMP-SP). The center proposed for the PhD sandwich is the Massachussets Institute of Technology (MIT-USA) which is a center of excellence in teaching and research in the area of this project. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

POLAK, ROBERTA; LIM, ROSANNA M.; BEPPU, MARISA M.; PITOMBO, RONALDO N. M.; COHEN, ROBERT E.; RUBNER, MICHAEL F.. Liposome-Loaded Cell Backpacks. ADVANCED HEALTHCARE MATERIALS, v. 4, n. 18, p. 2832-2841, DEC 30 2015. (11/21326-6)

POLAK, ROBERTA; BRADWELL, GRINIA M.; GILBERT, JONATHAN B.; DANIELSEN, SCOTT; BEPPU, MARISA M.; COHEN, ROBERT E.; RUBNER, MICHAEL F.. Optimization of Amine-Rich Multilayer Thin Films for the Capture and Quantification of Prostate-Specific Antigen. Langmuir, v. 31, n. 19, p. 5479-5488, MAY 19 2015. (11/21326-6)

POLAK, ROBERTA; CROUZIER, THOMAS; LIM, ROSANNA M.; RIBBECK, KATHARINA; BEPPU, MARISA M.; PITOMBO, RONALDO N. M.; COHEN, ROBERT E.; RUBNER, MICHAEL F.. Sugar-Mediated Disassembly of Mucin/Lectin Multi layers and Their Use as pH-Tolerant, On-Demand Sacrificial Layers. Biomacromolecules, v. 15, n. 8, p. 3093-3098, AUG 2014. (11/21326-6)