Topical application of liposomes containing cetuximab: the effect of physical skin penetration enhancement techniques for cutaneous squamous cell carcinoma

• Grant number: 12/23764-3
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): May 01, 2013
• Effective date (End): January 31, 2017
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: Renata Fonseca Vianna Lopez
• Grantee: Raquel Petrilli
• Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated scholarship(s): 13/15134-2 - Preparation of immunoliposomes for topical skin cancer treatment, BE.EP.DR

Abstract

Among skin cancers, the squamous cell carcinoma (SCC) helds the second position in frequency and is biologically more aggressive than the basocelular skin cancer. The topical chemotherapy of these tumors is a promising strategy for the reduction of the side effects associated to this kind of therapy. The cell pathway interruption using monoclonal antibodies, such as Cetuximab, is also a novel strategy for the inhibition of tumor growth. In this context, the present work aims at the use of Cetuximab for the treatment of SCC using for this purpose a topical drug delivery system based on liposomes. For this, we will use as a strategy to improve the penetration of the drug into the skin two physical methods, iontophoresis and sonophoresis. The systems will be developed containing encapsulated or covalently bond to Cetuximab and characterized for particle size, zeta potential, antibody integrity after conjugation and cytotoxicity for tumor cell line A431. Furthermore, the cutaneous and tumoral penetration will be assessed and the efficacy of the drug delivery systems developed will be investigated in vivo using an animal xenographic model for the disease using physical skin penetration enhancement techniques.

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PETRILLI, RAQUEL; ELOY, JOSIMAR O.; SAGGIORO, FABIANO P.; CHESCA, DEISE L.; DE SOUZA, MARINA CLARO; DIAS, MARCOS V. S.; DASILVA, LUIS L. P.; LEE, ROBERT J.; LOPEZ, RENATA F. V. Skin cancer treatment effectiveness is improved by iontophoresis of EGFR-targeted liposomes containing 5-FU compared with subcutaneous injection. JOURNAL OF CONTROLLED RELEASE, v. 283, p. 151-162, AUG 10 2018. Web of Science Citations: 5.

PETRILLO, R.; ELOY, J. O.; PASCHOAL, J. A. R.; LOPEZ, V, R. F. Quantification of 5-FU in skin samples for the development of new delivery systems for topical cancer treatment. Pharmazie, v. 73, n. 3, p. 133-138, MAR 2018. Web of Science Citations: 1.

PETRILLI, RAQUEL; ELOY, JOSIMAR O.; LOPEZ, RENATA F. V.; LEE, ROBERT J. Cetuximab Immunoliposomes Enhance Delivery of 5-FU to Skin Squamous Carcinoma Cells. ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY, v. 17, n. 2, p. 301-308, 2017. Web of Science Citations: 7.

ELOY, JOSIMAR O.; PETRILLI, RAQUEL; LOPEZ, RENATA F. V.; LEE, ROBERT J. Stimuli-Responsive Nanoparticles for siRNA Delivery. CURRENT PHARMACEUTICAL DESIGN, v. 21, n. 29, p. 4131-4144, 2015. Web of Science Citations: 10.

PETRILLI, RAQUEL; ELOY, JOSIMAR O.; MARCHETTI, JULIANA M.; LOPEZ, RENATA F. V.; LEE, ROBERT J. Targeted Lipid Nanoparticles for Antisense Oligonucleotide Delivery. CURRENT PHARMACEUTICAL BIOTECHNOLOGY, v. 15, n. 9, p. 847-855, 2014. Web of Science Citations: 9.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PETRILLI, Raquel. Topical application of liposomes containing cetuximab: effect of physical methods for skin penetration in skin squamous cell carcinoma. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto Ribeirão Preto.