Inactivation of Bacillus subtilis and Geobacillus stearothermophilus inoculated over metal surfaces using supercritical CO2 process and nisin

Autor(es)

da Silva, Mariana Altenhofen ^{[1, 2]} ; de Araujo, Amanda Piton ^[1] ; Ferreira, Juliana de Souza ^[3] ; Kieckbusch, Theo Guenter ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Chem Engn, Av Albert Einstein 500, BR-13083852 Campinas, SP - Brazil

^[2] Univ Fed Sao Carlos, Ctr Agr Sci, Rodovia Anhanguera, Km 174, BR-13600970 Araras, SP - Brazil

^[3] Univ Fed Uberlandia, Dept Chem Engn, Av Joao Naves Avila 2121, BR-38400 Uberlandia, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF SUPERCRITICAL FLUIDS; v. 109, p. 87-94, MAR 2016.
• Citações Web of Science: 6

Resumo

The use of supercritical CO2 (SC-CO2) as sterilizing agent of liquids and solid surfaces is arising as one of the most promising alternative sterilization techniques. This technology shows potential to substitute current chemical and thermal processes that, despite efficient, can damage the material. In this study, the effect of supercritical CO2 treatment (with and without ethanol as co-solvent) on the sterilization of Bacillus subtilis and Geobacillus stearothermophilus spores inoculated over metal surfaces were evaluated under pressure of 30 MPa and different temperatures (30 and 60 degrees C) and treatment times (60,120 and 180 min). The effects of pressure cycles (Delta P=30 MPa) and the addition of a natural antimicrobial agent (nisin) were also tested. Considering the tested conditions, SC-CO2 treatment alone was not able to efficiently reduce spore population of microorganisms. However, an interesting synergic sterilizing effect (more than 7 log cycles) was observed when SC-CO2 treatment was used in combination with low concentrations of nisin. (C) 2015 Published by Elsevier B.V. (AU)

• Processo FAPESP: 09/15849-6 - Avaliação e otimização dos parâmetros de inativação de microorganismos em ambientes supercríticos visando a esterilização de implantes cirúrgicos
• Beneficiário: Mariana Altenhofen da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 09/02677-2 - Avaliação e otimização dos parâmetros de inativação de microorganismos em ambientes supercríticos visando à esterilização de implantes cirúrgicos
• Beneficiário: Theo Guenter Kieckbusch
• Modalidade de apoio: Auxílio à Pesquisa - Regular