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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Inactivation of Bacillus subtilis and Geobacillus stearothermophilus inoculated over metal surfaces using supercritical CO2 process and nisin

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Author(s):
da Silva, Mariana Altenhofen [1, 2] ; de Araujo, Amanda Piton [1] ; Ferreira, Juliana de Souza [3] ; Kieckbusch, Theo Guenter [1]
Total Authors: 4
Affiliation:
[1] Univ Estadual Campinas, Sch Chem Engn, Av Albert Einstein 500, BR-13083852 Campinas, SP - Brazil
[2] Univ Fed Sao Carlos, Ctr Agr Sci, Rodovia Anhanguera, Km 174, BR-13600970 Araras, SP - Brazil
[3] Univ Fed Uberlandia, Dept Chem Engn, Av Joao Naves Avila 2121, BR-38400 Uberlandia, MG - Brazil
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF SUPERCRITICAL FLUIDS; v. 109, p. 87-94, MAR 2016.
Web of Science Citations: 6
Abstract

The use of supercritical CO2 (SC-CO2) as sterilizing agent of liquids and solid surfaces is arising as one of the most promising alternative sterilization techniques. This technology shows potential to substitute current chemical and thermal processes that, despite efficient, can damage the material. In this study, the effect of supercritical CO2 treatment (with and without ethanol as co-solvent) on the sterilization of Bacillus subtilis and Geobacillus stearothermophilus spores inoculated over metal surfaces were evaluated under pressure of 30 MPa and different temperatures (30 and 60 degrees C) and treatment times (60,120 and 180 min). The effects of pressure cycles (Delta P=30 MPa) and the addition of a natural antimicrobial agent (nisin) were also tested. Considering the tested conditions, SC-CO2 treatment alone was not able to efficiently reduce spore population of microorganisms. However, an interesting synergic sterilizing effect (more than 7 log cycles) was observed when SC-CO2 treatment was used in combination with low concentrations of nisin. (C) 2015 Published by Elsevier B.V. (AU)

FAPESP's process: 09/02677-2 - Evaluation and optimization of the process parameters that affect the inativation of microorganisms in supercritical ambients applied to the sterilization of biomedical implants
Grantee:Theo Guenter Kieckbusch
Support type: Regular Research Grants
FAPESP's process: 09/15849-6 - Evaluation and optimization of the process parameters that affect the inativation of microorganisms in supercritical ambients applied to the sterilization of biomedical implants
Grantee:Mariana Altenhofen da Silva
Support type: Scholarships in Brazil - Post-Doctorate