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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Spectral Sensitivity Measured with Electroretinogram Using a Constant Response Method

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Autor(es):
de Farias Rocha, Fernando Allan [1] ; Gomes, Bruno Duarte [2, 1] ; de Lima Silveira, Luiz Carlos [2, 3, 1] ; Martins, Sonia Limara [4] ; Aguiar, Renata Genaro [4] ; de Souza, John Manuel [4] ; Ventura, Dora Fix [4]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Fed Univ Para, Inst Ciencias Biol, BR-66059 Belem, Para - Brazil
[2] Fed Univ Para, Nucleo Med Trop, BR-66059 Belem, Para - Brazil
[3] Univ CEUMA, Sao Luis, Maranhao - Brazil
[4] Univ Sao Paulo, Inst Psicol, Sao Paulo - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 11, n. 1 JAN 22 2016.
Citações Web of Science: 0
Resumo

A new method is presented to determine the retinal spectral sensitivity function S(A) using the electroretinogram (ERG). S(A)s were assessed in three different species of myomorph rodents, Gerbils (Meriones unguiculatus), Wistar rats (Ratus norvegicus), and mice (Mus musculus). The method, called AC Constant Method, is based on a computerized automatic feedback system that adjusts light intensity to maintain a constant-response amplitude to a flickering stimulus throughout the spectrum, as it is scanned from 300 to 700 nm, and back. The results are presented as the reciprocal of the intensity at each wavelength required to maintain a constant peak to peak response amplitude. The resulting S(A) had two peaks in all three rodent species, corresponding to ultraviolet and M cones, respectively: 359 nm and 511 nm for mice, 362 nm and 493 nm for gerbils, and 362 nm and 502 nm for rats. Results for mouse and gerbil were similar to literature reports of S(A) functions obtained with other methods, confirming that the ERG associated to the AC Constant-Response Method was effective to obtain reliable S(A) functions. In addition, due to its fast data collection time, the AC Constant Response Method has the advantage of keeping the eye in a constant light adapted state. (AU)

Processo FAPESP: 02/12733-8 - Perdas sensoriais causadas pela intoxicação mercurial: estudos psicofísicos e neurofisiológicos em pacientes e em modelos animais
Beneficiário:Dora Selma Fix Ventura
Linha de fomento: Auxílio à Pesquisa - Temático