A new homoleptic coordination compound of ruthenium and norfloxacin and its interaction with human serum albumin

Autor(es)

Claro Reis, Felipe Costa ^[1] ; Viana Coelho, Joana Stefani ^[1] ; Pazin, Wallance Moreira ^[2] ; Nikolaou, Sofia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Av Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Fis, Av Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Inorganic Chemistry Communications; v. 63, p. 96-100, JAN 2016.
• Citações Web of Science: 4

Resumo

This work presents a new coordination compound of ruthenium and the norfloxacin antibiotics, as well as its interaction with human serum albumin (HSA). The ESI HRMS spectrum of {[}Ru(nor)(3)] (nor = norfloxacinate, C16H17FN3O3-) displayed the protonated molecular ion at m/z 1057.3, while the HRMS/MS spectra displayed peaks at m/z 958.4 and 639.3 assigned to the gaseous phase norfloxacin adducts (Hnor)(3).H+ and (Hnor)(2).H+. Coordination to Ru(III) remarkably enhances the water solubility of norfloxacin. The complex absorption spectrum in aqueous medium is dominated by the norfloxacin transitions in the UV region but also displays a broad shoulder around 370 nm, which possibly overlaps with ligand-field transitions. Stern-Volmer analysis of human serum albumin (HSA) fluorescence quenching by {[}Ru(nor)(3)] showed that both dynamic and static mechanisms contribute to the quenching. Lifetime measurements demonstrated that the predominance of one pathway over the other depends on temperature variations. Finally, the analysis of thermodynamic parameters leads to the conclusions that the interaction of the novel complex with HSA is spontaneous and that this interaction occurs through the formation of hydrogen bonds and van der Weals forces (Delta H = -259.2 kJ/mol and Delta S = -779.9 kJ/mol.K). (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 12/23245-6 - Espécies liberadoras de óxido nítrico baseadas em carboxilatos de rutênio e suas interações com algumas biomoléculas de interesse
• Beneficiário: Sofia Nikolaou
• Modalidade de apoio: Auxílio à Pesquisa - Regular