Alternagin-C (ALT-C), a disintegrin-like protein from Rhinocerophis alternatus snake venom promotes positive inotropism and chronotropism in fish heart

Autor(es)

Monteiro, D. A. ^[1] ; Kalinin, A. L. ^[1] ; Selistre-de-Araujo, H. S. ^[1] ; Vasconcelos, E. S. ^[1] ; Rantin, F. T. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Physiol Sci, BR-13560 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Toxicon; v. 110, p. 1-11, FEB 2016.
• Citações Web of Science: 4

Resumo

Alternagin-C (ALT-C) is a disintegrin-like protein purified from the venom of the snake, Rhinocerophis alternatus. Recent studies showed that ALT-C is able to induce vascular endothelial growth factor (VEGF) expression, endothelial cell proliferation and migration, angiogenesis and to increase myoblast viability. This peptide, therefore, can play a crucial role in tissue regeneration mechanisms. The aim of this study was to evaluate the effects of a single dose of alternagin-C (0.5 mg kg(-1), via intra-arterial) on in vitro cardiac function of the freshwater fish traira, Hoplias malabaricus, after 7 days. ALT-C treatment increased the cardiac performance promoting: 1) significant increases in the contraction force and in the rates of contraction and relaxation with concomitant decreases in the values of time to the peak tension and time to half- and 90% relaxation; 2) improvement in the cardiac pumping capacity and maximal electrical stimulation frequency, shifting the optimum frequency curve upward and to the right; 3) increases in myocardial VEGF levels and expression of key Ca2+-cycling proteins such as SERCA (sarcoplasmic reticulum Ca2+-ATPase), PLB (phospholamban), and NCX (Na+/Ca2+ exchanger); 4) abolishment of the typical negative force frequency relationship of fish myocardium. In conclusion, this study indicates that ALT-C improves cardiac function, by increasing Ca2+ handling efficiency leading to a positive inotropism and chronotropism. The results suggest that ALT-C may lead to better cardiac output regulation indicating its potential application in therapies for cardiac contractile dysfunction. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 13/00798-2 - A matriz extracelular no envelhecimento, no exercício e no microambiente tumoral
• Beneficiário: Heloisa Sobreiro Selistre de Araújo
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 12/10993-4 - Efeitos da alternagina-C (ALT-C), uma proteína extraída do veneno da serpente Urutu (Rhinocerophis alternatus, antiga Bothrops alternatus), sobre a contratilidade cardíaca de peixe em normóxia e frente à hipóxia tecidual
• Beneficiário: Diana Amaral Monteiro
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado