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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Alternagin-C (ALT-C), a disintegrin-like protein from Rhinocerophis alternatus snake venom promotes positive inotropism and chronotropism in fish heart

Texto completo
Autor(es):
Monteiro, D. A. [1] ; Kalinin, A. L. [1] ; Selistre-de-Araujo, H. S. [1] ; Vasconcelos, E. S. [1] ; Rantin, F. T. [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Fed Sao Carlos, Dept Physiol Sci, BR-13560 Sao Carlos, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Toxicon; v. 110, p. 1-11, FEB 2016.
Citações Web of Science: 4
Resumo

Alternagin-C (ALT-C) is a disintegrin-like protein purified from the venom of the snake, Rhinocerophis alternatus. Recent studies showed that ALT-C is able to induce vascular endothelial growth factor (VEGF) expression, endothelial cell proliferation and migration, angiogenesis and to increase myoblast viability. This peptide, therefore, can play a crucial role in tissue regeneration mechanisms. The aim of this study was to evaluate the effects of a single dose of alternagin-C (0.5 mg kg(-1), via intra-arterial) on in vitro cardiac function of the freshwater fish traira, Hoplias malabaricus, after 7 days. ALT-C treatment increased the cardiac performance promoting: 1) significant increases in the contraction force and in the rates of contraction and relaxation with concomitant decreases in the values of time to the peak tension and time to half- and 90% relaxation; 2) improvement in the cardiac pumping capacity and maximal electrical stimulation frequency, shifting the optimum frequency curve upward and to the right; 3) increases in myocardial VEGF levels and expression of key Ca2+-cycling proteins such as SERCA (sarcoplasmic reticulum Ca2+-ATPase), PLB (phospholamban), and NCX (Na+/Ca2+ exchanger); 4) abolishment of the typical negative force frequency relationship of fish myocardium. In conclusion, this study indicates that ALT-C improves cardiac function, by increasing Ca2+ handling efficiency leading to a positive inotropism and chronotropism. The results suggest that ALT-C may lead to better cardiac output regulation indicating its potential application in therapies for cardiac contractile dysfunction. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 13/00798-2 - A matriz extracelular no envelhecimento, no exercício e no microambiente tumoral
Beneficiário:Heloisa Sobreiro Selistre de Araújo
Modalidade de apoio: Auxílio à Pesquisa - Temático
Processo FAPESP: 12/10993-4 - Efeitos da alternagina-C (ALT-C), uma proteína extraída do veneno da serpente Urutu (Rhinocerophis alternatus, antiga Bothrops alternatus), sobre a contratilidade cardíaca de peixe em normóxia e frente à hipóxia tecidual
Beneficiário:Diana Amaral Monteiro
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado