Tuning Structural Changes in Glucose Oxidase for Enzyme Fuel Cell Applications

Autor(es)

Mecheri, Barbara ^[1] ; De Porcellinis, Diana ^[1] ; Campana, Patricia T. ^[2] ; Rainer, Alberto ^[3] ; Trombetta, Marcella ^[3] ; Marletta, Alexandre ^[4] ; Oliveira, Jr., Osvaldo N. ^[5] ; Licoccia, Silvia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Roma Tor Vergata, Dept Chem Sci & Technol, Via Ric Sci, I-00133 Rome - Italy

^[2] Univ Sao Paulo, Sch Arts Sci & Humanities, BR-03828000 Sao Paulo, SP - Brazil

^[3] Univ Campus Biomed Roma, I-00128 Rome - Italy

^[4] Univ Fed Uberlandia, Inst Phys, BR-38408100 Uberlandia, MG - Brazil

^[5] Univ Sao Paulo, Sao Carlos Inst Phys, BR-13560970 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ACS APPLIED MATERIALS & INTERFACES; v. 7, n. 51, p. 28311-28318, DEC 30 2015.
• Citações Web of Science: 5

Resumo

Stabilization and electrical contacting of redox enzymes with electrodes are fundamental requirements for bioelectronics devices, including biosensors and enzyme fuel cells (EFCs). In this study, we show increased glucose oxidase (GOx) stability by immobilization with Nafion. The immobilization process affected GOx conformation but was not detrimental to its activity, which was maintained for more than 120 days. The GOx/Nafion system was interfaced to a carbon cloth electrode and assembled in a prototypal EFC fed with glucose. Polarization and power density curves demonstrated that GOx/Nafion system was able to generate power, exploiting a Nafion-assisted electron transfer process to the electrode. Our findings are consistent with the onset of pH-dependent conformational equilibrium for the enzyme secondary structure and its active site. Significantly, the protective effect exerted by Nafion on the enzyme structure may be tuned by varying parameters such as the pH to fabricate durable EFCs with good electrocatalytic performance. (AU)

• Processo FAPESP: 12/13596-6 - Espectroscopia de fluorescência em estudos de estrutura e função de biomoléculas e suas aplicações no desenvolvimento de biomateriais.
• Beneficiário: Patricia Targon Campana
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/14262-7 - Filmes nanoestruturados de materiais de interesse biológico
• Beneficiário: Osvaldo Novais de Oliveira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático