Analytical development of a binuclear oxo-manganese complex bio-inspired on oxidase enzyme for doping control analysis of acetazolamide

Autor(es)

Machini, Wesley B. S. ^[1] ; Teixeira, Marcos F. S. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Fac Sci & Technol, Dept Chem & Biochem, Roberto Simonsen St 305, BR-19060900 Presidente Prudente, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOSENSORS & BIOELECTRONICS; v. 79, p. 442-448, MAY 15 2016.
• Citações Web of Science: 7

Resumo

A bio-inspired electrochemical sensor using a binuclear oxo-manganese complex was evaluated and applied in the detection of a substance associated with doping in sports: acetazolamide (ACTZ). Investigation was made of the influence of different experimental variables on the electrocatalytic oxidation of ACTZ by the bio-inspired sensor, such as pH and interfering species. The bio-inspired sensor showed the best response in the range from 5.00 x 10(-9) to 7.00 x 10(-8) mol L-1 ACTZ, with a linear range from 5.00 x 10(-9) to 2.50 x 10(-8) mol L-1 and a detection limit of 4.76 x 10(-9) mol L-1. The sensor exhibited characteristics similar to the Michaelis-Menten model of an enzymatic electrode, due to the use of a multinucleated complex of manganese with mu-oxo units, which was able to mimic the properties of enzymes with manganese as a cofactor in their composition, such as Mn-containing oxidase. The determination of ACTZ with the bio-inspired sensor was evaluated using three different synthetic biological fluids (plasma, saliva, and urine), demonstrating its viability for use with real samples. The analysis of ACTZ in real urine samples using the bio-inspired sensor, simulating the method adopted by the World Anti-Doping Agency, which revealed viable, suggesting a new and promising platform to be used in these analysis. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 05/01296-4 - Desenvolvimento de metodologia analítica empregando eletrodos modificados com oxo complexos polinucleares de rutênio encapsulado em zeolita
• Beneficiário: Marcos Fernando de Souza Teixeira
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 13/10469-6 - Aplicação eletroanalítica de complexo biomimetizador de manganês(IV) contendo ligante 2,3,5,6-tetraquis(2-piridil)pirazina
• Beneficiário: Wesley Bruno da Silva Machini
• Modalidade de apoio: Bolsas no Brasil - Mestrado